TP0300
Total Protein Kit, Micro Lowry, Peterson′s Modification
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About This Item
NACRES:
NA.32
UNSPSC Code:
12352202
usage
sufficient for ~200 assays
Quality Level
General description
This method utilizes sodium dodecylsulfate, included in the Lowry reagent, to facilitate the dissolution of relatively insoluble lipoproteins. An optional deoxycholate-TCA precipitation step permits removal of interfering substances such as Tris, sucrose, EDTA, salts, etc.
Application
Total Protein Kit, Micro Lowry, Peterson′s Modification has been used to measure protein content.
Biochem/physiol Actions
An optional deoxycholate-TCA precipitation step permits removal of interfering substances such as Tris, sucrose, EDTA, salts, etc. Absorbance at 500-800 nm (typically read at 750 nm) is used to determine protein concentration from a standard curve.
Features and Benefits
- Complete kit for protein assays
- Most widely cited protein assay
- Less protein to protein variation than other assays
- Contains reagents that can remove interfering substances
signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1 - STOT SE 3
target_organs
Respiratory system
Storage Class
8A - Combustible corrosive hazardous materials
wgk
WGK 3
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Protocols
To determine protein content, the Warburg-Christian method refers to measuring protein samples at 280 nm using a spectrophotometer.
Related Content
Products for traditional and alternative protein quantitation techniques available, including BCA, Bradford, Lowry, and more.
다양한 샘플 내의 단백질 농도를 정확히 측정하기 위한 단백질 정량법, 시약 및 면역분석 기술.