CAPHOS
Calcium Phosphate Transfection Kit
Most cost effective transfection reagent kit for transient and stable transfection of DNA into mammalian cells
Synonym(s):
Gene delivery
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About This Item
UNSPSC Code:
12352200
EC Number:
233-140-8
NACRES:
NA.85
Form:
solution
Grade:
Molecular Biology
Technique(s):
transfection: suitable
grade
Molecular Biology
Quality Segment
form
solution
usage
kit sufficient for 160 transfections (6 cm dishes), kit sufficient for 400 transfections (3.5 cm dishes), kit sufficient for 80 transfections (10 cm dishes)
technique(s)
transfection: suitable
shipped in
dry ice
storage temp.
−20°C
General description
Calcium phosphate transfection is a commonly used method for the introduction of DNA into eukaryotic cells. This technique has been used to obtain both transient and stable transfections in a wide variety of cell types.
Application
Suitable for transient and stable transfection of DNA into cultured mammalian cells. The following cells have successfully been transfected using the calcium phosphate method:
BAEC
Bowels melanoma cells
CHO K1
COS-7
Fibroblasts (human embryonic, neo derm)
HEK293
Huh 7
IMR-90
LLC (Lewis Lung Carcinoma)
NIH3T3
PC-12
PCI-13
SH-Sy5Y
SK-Hep-1
T47D
BAEC
Bowels melanoma cells
CHO K1
COS-7
Fibroblasts (human embryonic, neo derm)
HEK293
Huh 7
IMR-90
LLC (Lewis Lung Carcinoma)
NIH3T3
PC-12
PCI-13
SH-Sy5Y
SK-Hep-1
T47D
Calcium Phosphate Transfection Kit has been used:
- to enable transfection
- to transfect Hek293T cells
- to transfect H29D cells
Biochem/physiol Actions
The procedure is based on slow mixing of HEPES-buffered saline containing sodium phosphate with a CaCl2 solution containing the DNA. A DNA-calcium phosphate co-precipitate forms, which adheres to the cell surface and is taken up by the cell, presumably by endocytosis.
Features and Benefits
- Suitable for transient and stable transfection
- Reproducible for a wide range of cell types
- Widely referenced
- Inexpensive
Other Notes
The Calcium Phosphate Transfection Kit contains:
5 ml 2.5M CaCl2 (C2052)
25 ml 2x HEPES Buffered Saline (H1012)
25 ml molecular biology grade water (W4502)
5 ml 2.5M CaCl2 (C2052)
25 ml 2x HEPES Buffered Saline (H1012)
25 ml molecular biology grade water (W4502)
The protocol was developed for transient transfection of CHO cells using pSV40-CAT plasmid as a reporter gene.
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2
Storage Class
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 1
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Find documentation for the products that you have recently purchased in the Document Library.
A new technique for the assay of infectivity of human adenovirus 5 DNA.
F L Graham et al.
Virology, 52(2), 456-467 (1973-04-01)
A Calcium Phosphate Transfection
Ausubel, F. et al (ed.)
Short Protocols in Molecular Biology, 3rd, 9-9 (1995)
Munjin Kwon et al.
Methods in molecular biology (Clifton, N.J.), 1018, 107-110 (2013-05-18)
The calcium phosphate transfection is a widely used method for introducing foreign DNA plasmids into cells. Mechanisms underlying this transfection method are not yet defined; however, DNA-calcium phosphate precipitates are internalized by the cells and DNA is efficiently expressed in