D9281
Lambda DNA EcoR I Hind III Digest
for DNA electrophoresis
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About This Item
NACRES:
NA.25
UNSPSC Code:
41141827
Form:
liquid
Storage temp.:
−20°C
General description
Sigma′s Lambda DNA EcoR I Hind III Digest contains 13 fragments from 125 – 21,226 bp. Ten ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.
Application
Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.
Lambda DNA EcoR I Hind III Digest has been used as DNA markers for comparing the molecular weights of polymerase chain reaction (PCR) amplified products.
Preparation Note
Bacteriophage lambda DNA was digested with EcoR I and Hind III to completion, then purified by phenol extraction.
Other Notes
Contains 13 fragments, 125–21,226 bp
For optimal resolution, the recommended agarose gel concentration is 0.7%.
Sigma′s Lambda DNA EcoR I Hind III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.
signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1
Storage Class
13 - Non Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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