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L3037

Escort^™ III Transfection Reagent

Name: Escort<SUP>™</SUP> III Transfection Reagent
Brand: SIGMA

Lipid reagent for transfecting sensitive and primary cells

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About This Item

NACRES:

NA.25

UNSPSC Code:

41106502

Form:

liquid (aqueous solution)

Grade:

Molecular Biology

Technique(s):

transfection: suitable

Concentration:

1 mg/mL

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Properties

grade

Molecular Biology

Quality Level

200

form

liquid (aqueous solution)

usage

mL sufficient for 250-1000 transfections

concentration

1 mg/mL

technique(s)

transfection: suitable

storage temp.

2-8°C

Description

General description

Escort^™ III is a unique formulation of a proprietary polycationic lipid and a neutral non-transfecting lipid. This liposome-forming compound is used for transfection of nucleic acids into primary cells.

Application

Suitable for transient and stable transfection of nucleic acids into cultured eukaryotic cells. Use approximately 2-8 μl Escort^™ III and 2 μg DNA per 6 cm cell culture plate. Protocol optimization provides very efficient transfection. The following cells have been successfully transfected using Escort^™ III:

A549
C2C12 myotubes
Cardiomyocytes (rat)
COS-7
Fibroblasts (rat)
Germ cells (male rat)
Hepatocytes (rat and hamster)
HepG2
HeLa
Jurkat
Keratinocytes (human)
Myoblasts (mouse and quail)
Myocytes (mouse)
NIH3T3
PC-12
Retinal Neurons (rat)
Tracheobronchial cells (sheep)

Biochem/physiol Actions

A stable complex is formed when Escort^™ III is mixed with DNA in the absence of serum. The complexes are stable and can be directly added to the cell culture medium, where they fuse with the cell membrane, releasing the DNA into the cytoplasm. Note: complex formation is inhibited by serum, but once stable complexes have formed, the presence of serum is without consequence.

Features and Benefits

Suitable for stable and transient transfection
Optimized for a wide variety of primary cells
Low toxicity
Compatible with both serum and serum-free transfection protocols
Ideal for PC-12 cells

Other Notes

Escort^™ III formulation:
1 mg/mL total lipid in water

Note the identity of the lipids used in Escort^™ III is confidential.

Legal Information

Escort is a trademark of Sigma-Aldrich Co. LLC

Disclaimer

Do not freeze.

Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

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Protocols

Escort™ IV Transfection Reagent Protocol

Product manual provides detailed protocol for easy DNA transfection.

Escort™ III Transfection Reagent Protocol

The product bulletin providin detailed use protocol for easy DNA transfection.

Articles

Introduction to Cell Transfection

Transfection introduces genetic material into cells, aiding research in gene expression and cell biology.

How Transfection Works

This brief webinar provides an overview of what transfection is and the methods that are used to introduce DNA or RNA into eukaryotic cells.