T5574
Terrific Broth
Liquid microbial growth medium
Synonym(s):
TB medium, Tartoff – Hobbs medium
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About This Item
NACRES:
NA.85
UNSPSC Code:
41106200
grade
Molecular Biology
Quality Segment
description
Liquid Medium
sterility
sterile; 0.2 μm filtered
form
liquid
technique(s)
microbiological culture: suitable
application(s)
microbiology
storage temp.
room temp
suitability
nonselective for Escherichia coli, nonselective for coliforms
General description
Terrific Broth is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains glycerol, peptides, amino acids, water-soluble vitamins, and carbohydrates.
Application
Terrific Broth has been used for non-selective cultivation of E. coli strains for cloning, high-yield DNA plasmid production, and high-yield production of recombinant proteins. citation It is also suitable for selective cultivation when appropriate antibiotics are added.
Features and Benefits
Terrific Broth liquid provides:
- Filter-sterilized, ready-to-use format
- Convenient package size
- Standard formulation
Other Notes
23.6g/L Yeast Extract
11.8g/L Tryptone
9.4g/L K2HPO4
2.2g/L KH2PO4
4ml/L Glycerol
11.8g/L Tryptone
9.4g/L K2HPO4
2.2g/L KH2PO4
4ml/L Glycerol
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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