AB152

Anti-Tyrosine Hydroxylase Antibody

Name: Anti-Tyrosine Hydroxylase Antibody
Brand: MM

CHEMICON^®, rabbit polyclonal

동의어(들):

Tyrosine 3-hydroxylase, Tyrosine 3-monooxygenase, tyrosine hydroxylase, TH

조직 및 계약 가격을 보려면 로그인를 클릭합니다.

크기 선택

제품정보 (DICE 배송 시 비용 별도)

UNSPSC Code:

12352203

eCl@ss:

32160702

NACRES:

NA.41

Clone:

polyclonal

Species reactivity:

human, mollusk, feline, ferret, Aplysia (marine mollusc), rat, squid, Drosophila, mouse

Application:

ELISA, IF, IHC (p), IP, WB

Citations:

1045

주요 문서

모든 문서 보기

기술 서비스

도움이 필요하신가요? 저희 숙련된 과학자 팀이 도와드리겠습니다.

도움 문의

기술 서비스

도움이 필요하신가요? 저희 숙련된 과학자 팀이 도와드리겠습니다.

도움 문의

제품 이름

Anti-Tyrosine Hydroxylase Antibody, Chemicon^®, from rabbit

biological source

rabbit

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human, mollusk, feline, ferret, Aplysia (marine mollusc), rat, squid, Drosophila, mouse

manufacturer/tradename

Chemicon^®

technique(s)

ELISA: suitable, immunofluorescence: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunoprecipitation (IP): suitable, western blot: suitable

NCBI accession no.

NM_012740.3

UniProt accession no.

P04177

shipped in

dry ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... TH(7054)
rat ... Th(25085)

유사한 제품을 찾으십니까? 방문 제품 비교 안내

General description

62 kDa

Tyrosine hydroxylase (also know as Tyrosine 3-hydroxylase and Tyrosine 3-monooxygenase) plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of the catecholamines Dopamine and Norepinephrine from tyrosine. TH is, therefore, a useful marker for dopaminergic and noradrenergic neurons. The enzymatic activity of TH requires ferrous ions as cofactors and is believed to be regulated by phosphorylation. At least four isoforms of human TH have been identified which result from alternative splicing.

Immunogen

Denatured tyrosine hydroxylase from rat pheochromocytoma (denatured by sodium dodecyl sulfate).

Application

Anti-Tyrosine Hydroxylase Antibody detects level of TH and has been published and validated for use in ELISA, IF, IH, IH(P), IP and WB.

Immunohistochemistry:
A previous lot of this antibody was uesd at 1:100-1:1,000 dilution, 4°C, for 1-3 days. Stains both fresh frozen and paraffin embedded tissue samples by indirect immunofluorescence and immunoperoxidase.

Immunofluorescence:
A previous lot of this antibody was used at 1:100-1:1,000 dilution.

Immunoprecipitation:
A previous lot of this antibody was used on Immunoprecipitation.

ELISA:
A previous lot of this antibody was used in ELISA.

Optimal dilutions must be determined by the end user.

Research Category
Neuroscience

Research Sub Category
Neurotransmitters & Receptors

Neuronal & Glial Markers

Biochem/physiol Actions

Anti-Tyrosine Hydroxylase Antibodies react with most mammalian and many non-mammalian species. It has been reported that this antibody does not work on paraffin-embedded human tissue

Tyrosine hydroxylase. By western blot, AB152 selective labels a single band at approximately 62kDa (reduced) corresponding to Tyrosine Hydroxylase. It is expected that the antibody will react with most mammalian and many non-mammalian species. It has been reported that this antibody does not work on paraffin embedded human tissue.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing liquid in 10 mM HEPES (pH 7.5), 150 mM NaCl (pH 7.5), 100 ug/mL BSA and 50% glycerol.

Preparation Note

Maintain frozen at -20ºC to -70ºC in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. Do not store in a self defrosting freezer.

Analysis Note

Control
POSITIVE CONTROL: Brain (corpus striatum, sympathetic nerve terminals) and adrenal glands.
NEGATATIVE CONTROL: Liver.

Routinely evaluated by Western Blot on PC12 lysates.

Western Blot Analysis:
1:1000 dilution of this lot detected tyrosine hydroxylase on 10 μg of PC12 lysates.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

적합한 제품을 찾을 수 없으신가요?

당사의 제품 선택기 도구.을(를) 시도해 보세요.

저장 등급

10 - Combustible liquids

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

alpha-synuclein transgenic mice reveal compensatory increases in Parkinson's disease-associated proteins DJ-1 and Parkin and have enhanced alpha-synuclein and PINK1 levels after rotenone treatment.

George S, Mok SS, Nurjono M, Ayton S, Finkelstein DI, Masters CL, Li QX, Culvenor JG

Journal of Molecular Neuroscience null

Androgenic and oestrogenic influences on tyrosine hydroxylase-immunoreactive cells of the prairie vole medial amygdala and bed nucleus of the stria terminalis.

Cavanaugh BL, Lonstein JS

Journal of Neuroendocrinology null

Social novelty increases tyrosine hydroxylase immunoreactivity in the extended olfactory amygdala of female prairie voles.

Cavanaugh BL, Lonstein JS

Physiology & Behavior null

Foxa1 and Foxa2 regulate multiple phases of midbrain dopaminergic neuron development in a dosage-dependent manner.

Ferri, AL; Lin, W; Mavromatakis, YE; Wang, JC; Sasaki, H; Whitsett, JA; Ang, SL

Development null

Axon guidance of mouse olfactory sensory neurons by odorant receptors and the beta2 adrenergic receptor.

Feinstein, P; Bozza, T; Rodriguez, I; Vassalli, A; Mombaerts, P

Cell null

모든 관련된 서류 보기

문서

An Introduction to Antibodies: Antigens, Epitopes and Antibodies

Antibodies combine with specific antigens to generate an exclusive antibody-antigen complex. Learn about the nature of this bond and its use as a molecular tag for research.

Antibody Generation | Monoclonal vs Polyclonal Antibodies

Learn differences in monoclonal vs polyclonal antibodies including how antibodies are generated, clone numbers, and antibody formats.

Tips for Immunofluorescence Protocols

Immunofluorescence uses antibody-conjugated fluorescent molecules for protein localization, modification confirmation, and protein complex visualization.

Antibody Basics

Explore the basics of working with antibodies including technical information on structure, classes, and normal immunoglobulin ranges.

모두 보기

프로토콜

Tips and Techniques for Troubleshooting Immunohistochemistry (IHC)

Tips and troubleshooting for FFPE and frozen tissue immunohistochemistry (IHC) protocols using both brightfield analysis of chromogenic detection and fluorescent microscopy.

국제 무역 품목 번호

SKU	GTIN
AB152	04053252287138

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

고객지원팀으로 연락바랍니다.

Anti-Tyrosine Hydroxylase Antibody

CHEMICON®, rabbit polyclonal

크기 선택

제품정보 (DICE 배송 시 비용 별도)

주요 문서

속성

제품 이름

biological source

antibody form

antibody product type

clone

purified by

species reactivity

manufacturer/tradename

technique(s)

NCBI accession no.

UniProt accession no.

shipped in

target post-translational modification

Quality Level

Gene Information

설명

General description

Immunogen

Application

Biochem/physiol Actions

Physical form

Preparation Note

Analysis Note

Legal Information

Disclaimer

안전성 정보

저장 등급

문서

시험 성적서(COA)

이 제품을 이미 가지고 계십니까?

관련 참고 논문

관련 참고 프로토콜

문서

프로토콜

관련 콘텐츠

국제 무역 품목 번호

기술 서비스

CHEMICON^®, rabbit polyclonal