A8456
4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride
≥97.0% (HPLC)
동의어(들):
AEBSF
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제품정보 (DICE 배송 시 비용 별도)
실험식(Hill 표기법):
C8H10FNO2S · HCl
CAS 번호:
Molecular Weight:
239.69
UNSPSC Code:
12352202
NACRES:
NA.77
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
7604627
biological source
synthetic (organic)
Quality Segment
assay
≥97.0% (HPLC)
form
powder
solubility
H2O: 50 mg/mL
storage temp.
−20°C
SMILES string
FS(C1=CC=C(CCN)C=C1)(=O)=O.[H]Cl
InChI
1S/C8H10FNO2S.ClH/c9-13(11,12)8-3-1-7(2-4-8)5-6-10;/h1-4H,5-6,10H2;1H
InChI key
WRDABNWSWOHGMS-UHFFFAOYSA-N
General description
stable for up to six months if stored refrigerated at a pH of less than 7. If a pH of greater than 7 is required, pH adjustment should be made just prior to use.
Application
4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride (AEBSF) has been used:
- as a protease inhibitor in plasma samples to prevent acylated ghrelin (AG) degradation
- as a snake venom serine proteinase (SVSP) inhibitor in SVSP inhibition or AEBSF assay to test the involvement of SVSPs on the fibrinogen-clotting activity
- as a component in NP-40 lysis buffer to resuspend cell pellets for preparing cell lysates
Recommended concentrations for use are 0.1-1 mM.
Biochem/physiol Actions
4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride (AEBSF) is an irreversible serine protease inhibitor. Inhibition constants are similar to those of phenylmethanesulfonyl fluoride (PMSF) and diisopropylfluorophosphate (DFP). AEBSF has been shown to inhibit trypsin, chymotrypsin, plasmin kallikrein, and thrombin. As an alternative to PMSF and DFP, AEBSF offers lower toxicity, improved solubility in water, and improved stability in aqueous solutions. AEBSF can be used in cell culture in concentrations of up to 0.25 mM.
AEBSF can inhibit acetylcholinesterase (AChE).
signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1 - Skin Corr. 1A
저장 등급
8A - Combustible corrosive hazardous materials
flash_point_f
Not applicable
flash_point_c
Not applicable