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Merck

93350

Trizma® base

99.7% (T), puriss. p.a.

동의어(들):

2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol

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제품정보 (DICE 배송 시 비용 별도)

Linear Formula:
NH2C(CH2OH)3
CAS 번호:
77-86-1
Molecular Weight:
121.14
NACRES:
NA.25
PubChem Substance ID:
57653537
UNSPSC Code:
12352104
EC Number:
201-064-4
MDL number:
MFCD00004679
Beilstein/REAXYS Number:
741883

주요 문서

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도움 문의

description

aminopeptidase substrate

Quality Level

200

grade

puriss. p.a.

assay

99.7% (T)

form

crystalline

ign. residue

≤0.01% (as SO4)

pH

10.5-11.5 (25 °C, 10% in H2O)

useful pH range

7-9

pKa (25 °C)

8.1

bp

219-220 °C/10 mmHg (lit.)

mp

167-172 °C (lit.), 168-172 °C

solubility

water: soluble (678 g/l at 20 °C)

anion traces

chloride (Cl-): ≤5 mg/kg, sulfate (SO42-): ≤5 mg/kg

cation traces

Ca: ≤10 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, Fe: ≤5 mg/kg, K: ≤50 mg/kg, Mg: ≤5 mg/kg, Mn: ≤5 mg/kg, Na: ≤50 mg/kg, Ni: ≤5 mg/kg, Pb: ≤5 mg/kg, Zn: ≤5 mg/kg

λ

10 % in H2O

UV absorption

λ: 230 nm Amax: ≤0.6, λ: 260 nm Amax: ≤0.05, λ: 280 nm Amax: ≤0.04, λ: 430 nm Amax: ≤0.01

SMILES string

NC(CO)(CO)CO

InChI

1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2

InChI key

LENZDBCJOHFCAS-UHFFFAOYSA-N

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관련 카테고리

General description

Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.

Application

Tris base has been used:
  • In the preparation of TAE buffer for TAE agarose gel used to run PCR products by gel electrophoresis
  • As a component of sample buffer for SDS-PAGE
  • In the preparation of IEC (ion-exchange chromatography) buffer

Other Notes

Easily compare specifications for Trizma products with the Trizma specification table.
The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

저장 등급

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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시험 성적서(COA)

Lot/Batch Number

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문서 라이브러리 방문

Quantitative determination of titin and nebulin in poultry meat by SDS-PAGE with an internal standard.
Tomaszewska-Gras J, et al.
Meat Science, 62(1), 61-66 (2002)
a-Synuclein Aggregation Monitored by Thioflavin T Fluorescence Assay.
Wordehoff M and Hoyer W
Bio-protocol, 8(14) (2018)
In vivo Analysis of Cyclic di-GMP Cyclase and Phosphodiesterase Activity in Escherichia coli Using a Vc2 Riboswitch-based Assay.
Liu Y, et al.
Journal of Bacteriology (2017)
Tri (hydroxymethyl) aminomethane as Acidimetric Standard.
Fossum J, et al.
Analytical Chemistry, 23(3), 491-493 (1951)
Sambrook J and Russell DW
Molecular Cloning: A Laboratory Manual (2001)
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관련 콘텐츠

Trizma Base Specification Comparison

Trizma Base Specification Comparison

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