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Merck

D6546

DMEM - high glucose

With sodium Bicarbonate, sodium pyruvate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

동의어(들):

DME, Dulbecco′s Modified Eagle′s Medium - high glucose, DMEM

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제품정보 (DICE 배송 시 비용 별도)

UNSPSC Code:
12352207
NACRES:
NA.75
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제품 이름

Dulbecco′s Modified Eagle′s Medium - high glucose, With 4500 mg/L glucose, sodium pyruvate, and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Quality Segment

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

NaHCO3: yes
phenol red: yes
sodium pyruvate: yes
HEPES: no
L-glutamine: no
glucose: high

shipped in

ambient

storage temp.

2-8°C

General description

This DMEM-Hi glucose medium is a 1x complete medium with sodium pyruvate added. It also differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media. This medium requires supplementation with L-glutamine or L-alanyl-L-glutamine.

Application

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".

Preparation Note

Supplement with 0.584 g/L L-glutamine.


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pictograms

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signalword

Warning

hcodes

Hazard Classifications

Skin Sens. 1

저장 등급

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable



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