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T1503

Trizma^® base

Name: Trizma<SUP>&#174;</SUP> base
Brand: SIGMA

>=99.9% (titration), crystalline, primary standard, aminopeptidase substrate

동의어(들):

2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol

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제품정보 (DICE 배송 시 비용 별도)

Linear Formula:

NH₂C(CH₂OH)₃

CAS 번호:

77-86-1

Molecular Weight:

121.14

NACRES:

NA.25

PubChem Substance ID:

24899973

UNSPSC Code:

12352104

EC Number:

201-064-4

MDL number:

MFCD00004679

Beilstein/REAXYS Number:

741883

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속성

제품 이름

Trizma^® base, Primary Standard and Buffer, ≥99.9% (titration), crystalline

Quality Segment

300

description

aminopeptidase substrate

assay

≥99.9% (titration)

form

crystalline

storage condition

dry at room temperature

technique(s)

ELISA: suitable, protein extraction: suitable

color

white

pH

10.5-12

useful pH range

7-9

pKa (25 °C)

8.1

bp

219-220 °C/10 mmHg (lit.)

mp

167-172 °C (lit.)

solubility

methanol: soluble 26 mg/mL at 25 °C, ethylene glycol: soluble 79.1 mg/mL at 25 °C, water: soluble (678 g/l at 20 °C)

absorption

≤0.05 at 290 nm at 40%

suitability

suitable for Western blot, suitable for electrophoresis

application(s)

advanced drug delivery
cell analysis
diagnostic assay manufacturing
life science and biopharma

SMILES string

NC(CO)(CO)CO

InChI

1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2

InChI key

LENZDBCJOHFCAS-UHFFFAOYSA-N

설명

General description

Tris(hydroxymethyl)aminomethane, commonly known as Trometamol, Tris base, or Trizma^® base, serves a pivotal role in diverse research applications as a biological buffer. Its optimal pKa of 8.1 makes it a preferred choice for formulating buffers like Tris-acetate-EDTA (TAE) and Tris-borate-EDTA (TBE), ensuring the maintenance of pH within the physiological range (pH 7 - 9), applicable to a wide spectrum of living organisms. Despite its utility, researchers need to exercise caution in protein studies, as Tris has the potential to interfere with the activity of specific enzymes.

Tris base may find application as basimetric standard, independently as a buffer and as a crucial component in mixed buffer formulations, including Tris-EDTA (TE) buffer, TAE buffer, TBE buffer, among others.

Application

Trizma^® base has been used:

as a component of Hbuffer (cell dissociation buffer)
for washing and saturation of wells in double sandwich ELISA immunoenzymatic technique
as an assay buffer for reconstitution of extracted and dried protein samples
to prepare Tris-HCl buffer that is used to stabilize proteins
as a buffer to extract carotenoid from tubers
as a component of sample buffer during protein extraction prior to western blotting
as a component of sample buffer for sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)
to prepare simulated body fluid (SBF) for calcium phosphate (CaP) resorption assay
as a buffer for polydopamine (PDA) deposition on stainless steel (SS) substrate

Features and Benefits

Efficient buffering within the pH range of 7 - 9 with a pKa of 8.1 (25 °C)
Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
Can be used in Cell Biology, and Biochemical research

Packaging

The DRYPOUR^® packaging system for high-quality hygroscopic salts:

The current DRYPOUR^® packaging configuration is in a 50L nestable drum
25KG-DP are the standard prepack offering
Smaller volumes can be put in the same drum for variable configurations

The benefits of DRYPOUR^®:

Improves operational efficiency by reducing material preparation time and increasing operator safety
Minimizes caking of hygroscopic salts
Offers triple protection: against moisture inside and outside, and against contamination

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

For additional information on our range of Biochemicals, please complete this form.

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

DRYPOUR is a registered trademark of Merck KGaA, Darmstadt, Germany

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Tyvek is a registered trademark of E. I. du Pont de Nemours and Company

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저장 등급

11 - Combustible Solids

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Not applicable

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