A9434
Ammonium chloride
Molecular Biology, suitable for cell culture, ≥99.5%
동의어(들):
Salmiac
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크기 선택
제품정보 (DICE 배송 시 비용 별도)
Linear Formula:
NH4Cl
CAS 번호:
Molecular Weight:
53.49
NACRES:
NA.31
PubChem Substance ID:
eCl@ss:
38050207
UNSPSC Code:
12352302
EC Number:
235-186-4
MDL number:
Beilstein/REAXYS Number:
4371014
Assay:
≥99.5%
Grade:
Molecular Biology
Form:
powder or crystals
InChI key
NLXLAEXVIDQMFP-UHFFFAOYSA-N
InChI
1S/ClH.H3N/h1H;1H3
SMILES string
N.Cl
grade
Molecular Biology
vapor density
1.9 (vs air)
vapor pressure
1 mmHg ( 160.4 °C)
assay
≥99.5%
form
powder or crystals
technique(s)
cell culture | mammalian: suitable
mp
340 °C (subl.) (lit.)
anion traces
phosphate (PO43-): ≤2 ppm, sulfate (SO42-): ≤20 ppm
cation traces
Fe: ≤2 ppm, heavy metals (as Pb): ≤5 ppm
foreign activity
DNase, RNase, protease, none detected
Quality Level
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Application
Ammonium chloride has been used:
- as an inhibitor of endosome acidification in vero cells
- as an autophagy inhibitor in vascular smooth muscle cell (VSMC)
- in the depletion and lysis of erythrocytes in testis parenchyma homogenate
Biochem/physiol Actions
Ammonium chloride is one of the three principal components of the nitrogen cycle. Ammonium chloride administration promotes creatinine and urea clearance andinduces metabolic acidosis in mice. Excess of ammonium chloride inhibits Kreb′s pathway in brain and depletes ATP in astrocytes. Ammonium chloride inhibits acidification in the endosome-lysosome system. Ammonium chloride is a hemolysing agent used for the lysis of erythrocytes.
signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Eye Irrit. 2
저장 등급
13 - Non Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
Infectious bronchitis virus entry mainly depends on clathrin mediated endocytosis and requires classical endosomal/lysosomal system
Wang H, et al.
Virology, 528, 118-136 (2019)
Effect of ammonium chloride and dietary phosphorus in the azotaemic rat. I. Renal function and biochemical changes
Jara A, et al.
Nephrology, Dialysis, and Transplantation, 19(8), 1986-1992 (2004)
Nrf2 Activation Provides Atheroprotection in Diabetic Mice Through Concerted Upregulation of Antioxidant, Anti-inflammatory, and Autophagy Mechanisms
Lazaro I, et al.
Frontiers in Pharmacology, 9, 118-136 (2018)
Aqueous ammonia and ammonium chloride hydrates: Principal infrared spectra
Max JJ and Chapados C
Journal of Molecular Structure, 1046, 124-135 (2013)
Induction of metabolic acidosis with ammonium chloride (NH4Cl) in mice and rats-species differences and technical considerations
Nowik M, et al.
Cellular Physiology and Biochemistry, 26(6), 1059-1072 (2010)
프로토콜
The GenElute Mammalian Genomic DNA Purification Kit Protocol describes the isolation of pure, high molecular weight DNA from a variety of mammalian sources.
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