P7280
Poly-ᴅ-Lysine Hydrobromide
synthetic, mol wt 30,000-70,000, powder, γ-irradiated, suitable for cell culture, BioXtra
동의어(들):
PDL
조직 및 계약 가격을 보려면 로그인를 클릭합니다.
크기 선택
제품정보 (DICE 배송 시 비용 별도)
Linear Formula:
D-Lys-(D-Lys)n-D-Lys · xHBr
CAS 번호:
UNSPSC Code:
12352202
PubChem Substance ID:
NACRES:
NA.75
MDL number:
Biological source:
synthetic (organic)
Form:
lyophilized powder
Technique(s):
cell culture | mammalian: suitable
Concentration:
0.016—0.032 mmol lysine
Mol wt:
average mol wt 30,000-70,000
제품 이름
Poly-D-lysine hydrobromide, average mol wt 30,000-70,000, lyophilized powder, γ-irradiated, BioReagent, suitable for cell culture
SMILES string
O=C(C)[C@@](NC)([H])CCCCN.[Br]
InChI
1S/C6H14N2O2.BrH/c7-4-2-1-3-5(8)6(9)10;/h5H,1-4,7-8H2,(H,9,10);1H
InChI key
MEXAGTSTSPYCEP-UHFFFAOYSA-N
biological source
synthetic (organic)
sterility
γ-irradiated
product line
BioReagent, BioXtra
form
lyophilized powder
mol wt
average mol wt 30,000-70,000
packaging
pkg of 5 X 5 mg, pkg of 5 mg
concentration
0.016—0.032 mmol lysine
technique(s)
cell culture | mammalian: suitable
surface coverage
4 μg/cm2
solubility
H2O: soluble 50 mg/mL, clear, colorless
shipped in
ambient
storage temp.
−20°C
Quality Level
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General description
Poly-D-Lysine Hydrobromide (PDL), a positively charged polymer consisting of lysine residues, where each lysine unit is associated with a hydrobromide molecule. This hydrobromide molecule gives the poly-D-lysine a unique characteristic, enabling it to form crystals and making it easily soluble in water-based solutions.
Application
Poly d lysine hydrobromide coating has been used:
- In coating tissue culture plate wells and coverslips for immunofluorescence microscopy of primary neuronal culture to study neurological disorders associated with Zika virus infection.
- To coat the coverslips for enhancing the attachment and proliferation of the myenteric cells isolated from the rat intestines.
- In preparing surface of coverslips for cell attachment for studying the myenteric plexus of rat’s small intestine.
Poly-D-lysine polymers can be used in preparing surfaces for cell attachment. The D-lysine polymers can also be used with cells that digest poly-L-lysine polymers and cause an excessive uptake of L-lysine.
This product is recommended as a cell culture substratum when using 0.5 - 1.0 mL of a 0.1 mg/mL solution to coat 25 cm2. Lower molecular weight versions of the product are less viscous, but high more molecular weight versions provide more attachment sites per molecule.
This product is recommended as a cell culture substratum when using 0.5 - 1.0 mL of a 0.1 mg/mL solution to coat 25 cm2. Lower molecular weight versions of the product are less viscous, but high more molecular weight versions provide more attachment sites per molecule.
Biochem/physiol Actions
Poly-D-lysine (PDL) hydrobromide is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. After absorption to the culture surface, poly-D-lysine increases the number of positively charged cell binding sites.
Features and Benefits
Sterilized using γ-irradiation
Preparation Note
This product has a molecular weight of 30,000-70,000 and is cell culture tested and gamma-irradiated. To remove the HBr, dissolve this product in a neutral buffer and dialyze to remove the salts. In general, to use this product as an attachment factor, add 50 mL of sterile tissue culture grade water to 5 mg of poly-lysine, and aseptically coat the surface with 1 mL per 25 cm2 of solution. After 5 minutes, remove the solution through aspiration and thoroughly rinse the surface. Let dry for two hours before introducing cells and medium.
Other Notes
Poly-D-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-D-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the β structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.
Disclaimer
Sterile solutions are stable for up to 2 years when stored at 2-8°C. It should be stored desiccated at -20°C.
저장 등급
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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문서
Poly-Lysine enhances cell binding with positively-charged surface ions, optimizing electrostatic interaction on culture surfaces for increased cell attachment.
Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.
프로토콜
Adhere cells to solid substrates using poly-lysine, which enhances electrostatic interaction between negatively charged ions of the cell membrane and the culture surface.
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