T2885
3,3′,5,5′-Tetramethylbenzidine
TMB substrate, chromogenic, ≥98% (TLC), powder
동의어(들):
BM blue, Sure Blue TMB, TMB, TMB Blotting Plus, TMB substrate
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크기 선택
제품정보 (DICE 배송 시 비용 별도)
Linear Formula:
[-C6H2(CH3)2-4-NH2]2
CAS 번호:
Molecular Weight:
240.34
NACRES:
NA.83
PubChem Substance ID:
UNSPSC Code:
12352204
EC Number:
259-364-6
MDL number:
Beilstein/REAXYS Number:
2808541
제품 이름
3,3′,5,5′-Tetramethylbenzidine, ≥98% (TLC)
InChI key
UAIUNKRWKOVEES-UHFFFAOYSA-N
InChI
1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3
SMILES string
Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2
assay
≥98% (TLC)
form
powder
mp
168-171 °C (lit.)
solubility
ethyl acetate: 50 mg/mL, clear to very slightly hazy
storage temp.
2-8°C
Quality Level
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Application
3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin, assay of peroxidases.
3,3′,5,5′-Tetramethylbenzidine has been used:
- as a supplement in Man-Rodosa-Sharpe (MRS) agar plate, to stains the lactobacilli colonies blue in the presence of O2
- as a substrate solution for ELISA (enzyme-linked immunosorbent assay)
- in myeloperoxidase activity assay to quantify neutrophil
Biochem/physiol Actions
3,3′,5,5′-Tetramethylbenzidine/TMB can be used as a chromogen to increase the progression of product obtained in peroxidase reaction. In food and environmental decontamination procedures, TMB can be used in in situ free available chlorine (FAC) monitoring of chlorite-based sanitizers.
Packaging
Bottomless glass bottle. Contents are inside inserted fused cone.
signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Chronic 4 - Carc. 2
저장 등급
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
Ferrotransferrin and antibody against the transferrin receptor as potential vehicles for drug delivery across the mammalian blood-brain barrier into the central nervous system
Methods in Neurosciences, 21(1), 93-117 (1994)
The CD4+ T cell-mediated IFN-gamma response to Helicobacter infection is essential for clearance and determines gastric cancer risk
Sayi A, et al.
Journal of immunology (Baltimore, Md. : 1950), 182(11), 7085-7101 (2009)
Lactobacilli isolated from vaginal vault of dairy and meat cows during progesteronic stage of estrous cycle
Rodriguez C, et al.
Anaerobe, 17(1), 15-18 (2011)
Lactobacillus casei improves resistance to pneumococcal respiratory infection in malnourished mice
Villena J, et al.
The Journal of Nutrition, 135(6), 1462-1469 (2005)
Tetramethylbenzidine method for monitoring the free available chlorine and microbicidal activity of chlorite-based sanitizers under organic-matter-rich environments
Yamaoka H, et al.
Letters in Applied Microbiology, 62(1), 47-54 (2016)
문서
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
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