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제품정보 (DICE 배송 시 비용 별도)
Linear Formula:
NH2CONH2
CAS 번호:
Molecular Weight:
60.06
UNSPSC Code:
12352111
NACRES:
NA.31
PubChem Substance ID:
EC Number:
200-315-5
Beilstein/REAXYS Number:
635724
MDL number:
Assay:
≥98%
Grade:
Molecular Biology
Technique(s):
cell culture | mammalian: suitable
grade
Molecular Biology
Quality Level
product line
BioReagent
assay
≥98%
form
powder
technique(s)
cell culture | mammalian: suitable
mp
132-135 °C (lit.)
solubility
H2O: soluble 480 mg/mL, clear, colorless (8M)
density
1.335 g/mL at 25 °C (lit.)
anion traces
chloride (Cl-): ≤5 ppm
cation traces
Cu: ≤1 ppm, Fe: ≤5 ppm, Pb: ≤2 ppm
absorption
≤0.06 at 260 at 6 M, ≤0.06 at 280 at 6 M
foreign activity
DNase, NICKase, RNase and protease, none detected
SMILES string
NC(N)=O
InChI
1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)
InChI key
XSQUKJJJFZCRTK-UHFFFAOYSA-N
Gene Information
human ... CA1(759), CA2(760)
rat ... Ppm1a(24666)
Application
Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.
- Urea has been used in protein resuspension buffer and to denature proteins.
- It has been used for the isolation of proteins via urea-containing polyacrylamide gels.
- It has been used to study the conformation of unfolded proteins under different concentrations of urea and different pH.
저장 등급
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Labeling and identification of direct kinase substrates.
Carlson SM and White FM
Science Signaling, 5, pl3-pl3 (2012)
Construction and transfection of a ribozyme targeting human caspase-3.
Cronin D et al.
Anticancer Research, 24, 425-425 (2004)
Structure and properties of native and unfolded lysing enzyme from T. harzianum: Chemical and pH denaturation.
Bey H et al.
International Journal of Biological Macromolecules, 92, 860-860 (2016)