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Merck

U5378

Urea

powder, BioReagent, Molecular Biology, suitable for cell culture

동의어(들):

Carbamide, Carbonyldiamide

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제품정보 (DICE 배송 시 비용 별도)

Linear Formula:
NH2CONH2
CAS 번호:
Molecular Weight:
60.06
UNSPSC Code:
12352111
NACRES:
NA.31
PubChem Substance ID:
EC Number:
200-315-5
Beilstein/REAXYS Number:
635724
MDL number:
Assay:
≥98%
Grade:
Molecular Biology
Technique(s):
cell culture | mammalian: suitable
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grade

Molecular Biology

Quality Level

product line

BioReagent

assay

≥98%

form

powder

technique(s)

cell culture | mammalian: suitable

mp

132-135 °C (lit.)

solubility

H2O: soluble 480 mg/mL, clear, colorless (8M)

density

1.335 g/mL at 25 °C (lit.)

anion traces

chloride (Cl-): ≤5 ppm

cation traces

Cu: ≤1 ppm, Fe: ≤5 ppm, Pb: ≤2 ppm

absorption

≤0.06 at 260 at 6 M, ≤0.06 at 280 at 6 M

foreign activity

DNase, NICKase, RNase and protease, none detected

SMILES string

NC(N)=O

InChI

1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)

InChI key

XSQUKJJJFZCRTK-UHFFFAOYSA-N

Gene Information

human ... CA1(759), CA2(760)
rat ... Ppm1a(24666)

Application

Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.
  • Urea has been used in protein resuspension buffer and to denature proteins.
  • It has been used for the isolation of proteins via urea-containing polyacrylamide gels.
  • It has been used to study the conformation of unfolded proteins under different concentrations of urea and different pH.


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저장 등급

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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문서 라이브러리 방문



Labeling and identification of direct kinase substrates.
Carlson SM and White FM
Science Signaling, 5, pl3-pl3 (2012)
Construction and transfection of a ribozyme targeting human caspase-3.
Cronin D et al.
Anticancer Research, 24, 425-425 (2004)
Structure and properties of native and unfolded lysing enzyme from T. harzianum: Chemical and pH denaturation.
Bey H et al.
International Journal of Biological Macromolecules, 92, 860-860 (2016)