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About This Item
UNSPSC Code:
41104923
NACRES:
NB.22
eCl@ss:
32011202
Material:
transparent PTFE membrane, polystyrene housing (5 mm height)
Pore size:
0.4 μm
Sterility:
ethylene oxide treated, sterile
Binding type:
low binding surface
Quality Segment
material
transparent PTFE membrane, polystyrene housing (5 mm height)
sterility
ethylene oxide treated, sterile
feature
hydrophilic
manufacturer/tradename
Millicell®
packaging
pkg of Individually blister packaged
parameter
50 °C max. temp.
technique(s)
cell attachment: suitable, cell culture | mammalian: suitable, cell differentiation: suitable
H
5 mm
diam.
30 mm
size
6 wells
surface area
4.2 cm2
color
transparent membrane, when wetted
matrix
Biopore™
pore size
0.4 μm
binding type
low binding surface
detection method
fluorometric
shipped in
ambient
storage temp.
10-30°C
General description
With Millicell inserts, attachment or suspension cells can access media from both their apical and basolateral sides. Cell growth, structure, and function more closely mimic what occurs in vivo. In addition, Millicell inserts make it possible to study both sides of the cell monolayer.
Application
- Cell Attachment, 3D Cell Culture, Cell Growth, Cell Differentiation , Immunocytochemistry
- Biopore Membrane (hydrophilic PTFE) is ideal for low protein-binding, live cell viewing, and immunofluorescent applications
Features and Benefits
- For high cell viability and superior study of three dimensional explant structure
- Lower height allows them to fit inside a standard petri dish
- The Biopore (PTFE) membrane provides high viability—for as long as 40 days—and excellent trans-membrane oxygen transport
- The membrane is optically clear and optimized for long-term organotypic explant maintenance
Preparation Note
To ensure proper wetting of the membrane from the basolateral side and to mitigate a chance of bubble formation:
- Add assay solution or media to the receiver well: 2 mL for 6 well format orequivalent dimensions.
- With forceps, place the insert into the receiver well at a 30°-45° angle togradually wet it out from the basolateral side.
- Once edges start to wet out, gently lower the insert until it is fully seatedinto the well.
Legal Information
BIOPORE is a trademark of Merck KGaA, Darmstadt, Germany
Millicell is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
10-13 - German Storage Class 10 to 13
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