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14-759 Abl (Y253F) Protein, active, 10 µg

Active, N-terminal 6His-tagged recombinant human Abl, residues 27-end containing the Y253F mutation. For use in Kinase Assays.

Abl (Y253F) Protein, active, 10 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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14-759
10 µg  Also available in 250μg size as catalog # 14-759M - Call for pricing and availability.
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      Overview

      Replacement Information
      Description
      Catalogue Number14-759
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAbl (Y253F) Protein, active, 10 µg
      OverviewN-terminal 6His-tagged recombinant human Abl, residues 27-end containing the Y253F mutation.
      Background InformationThe Abl tyrosine kinase inhibitor STI571 is an effective therapy for stable-phase chronic myeloid leukemia patients. Many patients responding to STI571 later relapse due to a reactivation of Bcr-Abl activity. In certain cases this appears to correlate with the presence of the Y253F mutation, which confers resistance to drug binding (Shah et al., (2002), Cancer Cell 2, 117-125).
      References
      Product Information
      Presentation50mM Tris/HCl pH7.5, 150mM NaCl, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1mM EGTA, 0.1% 2-mercaptoethanol, 0.03% Brij 35. Frozen solution.
      Quality SegmentMQ100
      Applications
      ApplicationActive, N-terminal 6His-tagged recombinant human Abl, residues 27-end containing the Y253F mutation. For use in Kinase Assays.
      Key Applications
      • Kinase Assay
      Biological Information
      Source Expressed by baculovirus in Sf21 insect cells.
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Entrez Gene Number
      Gene Symbol
      • ABL1
      • JTK7
      • ABL
      • p150
      • vABL
      • cABL
      • bcr/abl
      Protein TargetAbl
      Purification MethodNi2+/NTA-agarose
      Target Sub-FamilyTK
      UniProt Number
      Molecular Weight121 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by phosphorylation of Abltide.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore -70°C. For maximum recovery of product, centrifuge original vial prior to removing the cap.
      Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.
      Packaging Information
      Material Size10 µg
      Material PackageAlso available in 250μg size as catalog # 14-759M - Call for pricing and availability.
      Transport Information
      Supplemental Information
      Specifications
      Global Trade ITEM Number
      Catalogue Number GTIN
      14-759 04053252731556