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203900 Bufalin - CAS 465-21-4 - Calbiochem

A cardiotonic steroid isolated from toads of Bufo species that potently inhibits ouabain-sensitive Na+,K+-ATPase activity (IC₅₀ = 1.4 nM).

Bufalin - CAS 465-21-4 - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: 5β, 20(22)-Bufadienolide-3β, 14-diol

Primary Target: Ouabain-sensitive Na+,K+-ATPase activity
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203900
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Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
465-21-4C₂₄H₃₄O₄

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      Description
      OverviewA cardiotonic steroid isolated from toads of Bufo species that potently inhibits ouabain-sensitive Na+,K+-ATPase activity (IC50 = 1.4 nM). Induces apoptosis in various human cell lines such as leukemia HL-60 and U937 cells by altering the expression of apoptosis-related genes. Reported to stimulate the activities of p21 activated kinase (PAK) and c-Jun Kinase (JNK) in leukemic cell lines. Induces a transient increase in the expression of Tiam1 mRNA in U937 cell lines (~100 nM). Bufalin also down-regulates gene expression of the intracellular signaling protein 14-3-3 in rat lens, probably a consequence of Na+,K+-ATPase inhibition. Reduces the level of topoisomerase II in human leukemia cells.
      Catalogue Number203900
      Brand Family Calbiochem®
      Synonyms5β, 20(22)-Bufadienolide-3β, 14-diol
      References
      ReferencesKurosawa, M., et al. 2000. Am. J. Physiol. 278, C500.
      Kawazoe, N., et al. 1999. Oncogene 18, 2413.
      McGowan, M.H., et al. 1999. J. Pharmacol. Exp. Ther. 289, 1559.
      Watabe, M., et al. 1998. Oncogene 16, 779.
      Hashimoto, S., et al. 1997. Leuk. Res. 21, 875.
      Morris, J.F., et al. 1997. Comp. Biochem. Physiol. A Physiol. 118, 599.
      Brownlee, A.A., et al. 1990. Clin. Sci. 78, 169.
      Product Information
      CAS number465-21-4
      ATP CompetitiveN
      FormWhite to off-white solid
      Hill FormulaC₂₄H₃₄O₄
      Chemical formulaC₂₄H₃₄O₄
      HS Code2937 29 90
      ReversibleN
      Structure formula ImageStructure formula Image
      Quality SegmentMQ100
      Applications
      Biological Information
      Primary TargetOuabain-sensitive Na+,K+-ATPase activity
      Primary Target IC<sub>50</sub>1.4 nM against ouabain-sensitive Na+,K+-ATPase activity
      Purity≥98% by HPLC
      Physicochemical Information
      Cell permeableN
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      RTECSEI2962500
      Safety Information
      R PhraseR: 26/27/28

      Very toxic by inhalation, in contact with skin and if swallowed.
      S PhraseS: 22-36/37/39-45

      Do not breathe dust.
      Wear suitable protective clothing, gloves and eye/face protection.
      In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Highly Toxic
      Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
      Storage -20°C
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications
      Global Trade ITEM Number
      Catalogue Number GTIN
      203900-10MG 04055977219937

      Documentation

      Bufalin - CAS 465-21-4 - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      Bufalin - CAS 465-21-4 - Calbiochem Certificates of Analysis

      TitleLot Number
      203900

      References

      Reference overview
      Kurosawa, M., et al. 2000. Am. J. Physiol. 278, C500.
      Kawazoe, N., et al. 1999. Oncogene 18, 2413.
      McGowan, M.H., et al. 1999. J. Pharmacol. Exp. Ther. 289, 1559.
      Watabe, M., et al. 1998. Oncogene 16, 779.
      Hashimoto, S., et al. 1997. Leuk. Res. 21, 875.
      Morris, J.F., et al. 1997. Comp. Biochem. Physiol. A Physiol. 118, 599.
      Brownlee, A.A., et al. 1990. Clin. Sci. 78, 169.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision22-May-2008 RFH
      Synonyms5β, 20(22)-Bufadienolide-3β, 14-diol
      DescriptionA cardiotonic steroid isolated from Bufo species toads that potently inhibits ouabain-sensitive Na+,K+-ATPase activity. Induces apoptosis in various human cell lines such as leukemia HL60 and U937 cells by altering the expression of apoptosis-related genes. Reported to stimulate the activities of p21 activated kinase (PAK) and c-Jun Kinase (JNK) in leukemic cell lines. Induces a transient increase in the expression of Tiam1 mRNA in U937 cell lines (~100 nM). Bufalin also down-regulates gene expression of the intracellular signaling protein 14-3-3 in rat lens, probably a consequence of Na+-K+-ATPase inhibitors. Reduces the level of topoisomerase II in human leukemia cells.
      FormWhite to off-white solid
      Intert gas (Yes/No) Packaged under inert gas
      CAS number465-21-4
      RTECSEI2962500
      Chemical formulaC₂₄H₃₄O₄
      Structure formulaStructure formula
      Purity≥98% by HPLC
      SolubilityDMSO (25 mg/ml) or warm Ethanol (25 mg/ml)
      Storage Protect from light
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Toxicity Highly Toxic
      ReferencesKurosawa, M., et al. 2000. Am. J. Physiol. 278, C500.
      Kawazoe, N., et al. 1999. Oncogene 18, 2413.
      McGowan, M.H., et al. 1999. J. Pharmacol. Exp. Ther. 289, 1559.
      Watabe, M., et al. 1998. Oncogene 16, 779.
      Hashimoto, S., et al. 1997. Leuk. Res. 21, 875.
      Morris, J.F., et al. 1997. Comp. Biochem. Physiol. A Physiol. 118, 599.
      Brownlee, A.A., et al. 1990. Clin. Sci. 78, 169.