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14-444 PKC θ Protein, active, 10 µg

Active, recombinant full-length human PKC theta with a C-terminal 6His-tag. For use in Kinase Assays.

PKC θ Protein, active, 10 µg: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
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14-444
10 µg  
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      Descripción

      Replacement Information
      Description
      Catalogue Number14-444
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionPKC θ Protein, active, 10 µg
      OverviewRecombinant full-length human PKC theta with a C-terminal 6His-tag

      PKC Lipid activator (Catalog # 20-133) is required for use.
      References
      Product Information
      Quality SegmentMQ100
      Applications
      ApplicationActive, recombinant full-length human PKC theta with a C-terminal 6His-tag. For use in Kinase Assays.
      Key Applications
      • Kinase Assay
      Biological Information
      Sourceexpressed in Sf21 cells
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Entrez Gene Number
      Entrez Gene SummaryProtein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1, and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factors.
      Gene Symbol
      • PRKCQ
      • OTTHUMP00000043365
      • MGC126514
      • OTTHUMP00000043364
      • MGC141919
      • PRKCT
      • nPKC-theta
      Protein TargetPKCθ
      Purification Methodimmobilized nickel affinity chromatography
      Target Sub-FamilyAGC
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q04759 # PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
      COFACTOR: Magnesium.
      SIZE: 706 amino acids; 81865 Da
      SUBUNIT: Interacts with TXNL2/PICOT.
      TISSUE SPECIFICITY: Skeletal muscle, megakaryoblastic cells and platelets.
      DOMAIN: SwissProt: Q04759 The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.
      PTM: Autophosphorylation at Thr-219 is required for targeting to the TCR and cellular function of PKC upon antigen receptor ligation.
      SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 C2 domain. & Contains 2 phorbol-ester/DAG-type zinc fingers. & Contains 1 protein kinase domain.
      Molecular Weight83kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by phosphorylation of Histone H1 substrate in vitro
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions6 months at -70 °C
      Packaging Information
      Material Size10 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade ITEM Number
      Número de referencia GTIN
      14-444 04053252270130

      Documentation

      PKC θ Protein, active, 10 µg Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      PKC θ Protein, active, 10 µg Certificados de análisis

      CargoNúmero de lote
      PKC Theta, active - D7MN009U-H D7MN009U-H
      PKC Theta, active - D7MN009U-I D7MN009U-I
      PKC Theta, active - D7MN010U-D D7MN010U-D

      Referencias bibliográficas

      Visión general referenciasAplicación Pub Med ID
      Complex formation and cooperation of protein kinase C theta and Akt1/protein kinase B alpha in the NF-kappa B transactivation cascade in Jurkat T cells
      Bauer, B., et al
      J Biol Chem, 276:31627-34 (2001)  2001

      		Kinase Assay11410591 11410591
      Molecular cloning and characterization of PKC theta, a novel member of the protein kinase C (PKC) gene family expressed predominantly in hematopoietic cells.
      Baier, G, et al.
      J. Biol. Chem., 268: 4997-5004 (1993)  1992

      		8444877 8444877