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14-567 Pyk2 Protein, active, 10 µg

Active, N-Terminal His6-tagged, recombinant, full-length, human Pyk2. For use in Kinase Assays.

Pyk2 Protein, active, 10 µg: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
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14-567
10 µg  Also available in 250 µg size --call for pricing and availability and reference catalog number 14-567M when ordering the 250 µg size.
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      Descripción

      Replacement Information
      Description
      Catalogue Number14-567
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionPyk2 Protein, active, 10 µg
      OverviewN-Terminal His6-tagged, recombinant, full-length, human Pyk2.
      Alternate Names
      • Protein-tyrosine kinase 2-beta
      • Calcium-dependent tyrosine kinase
      • CADTK
      • Calcium-regulated non-receptor proline-rich tyrosine kinase
      • Cell adhesion kinase beta
      • CAK-beta
      • CAKB
      • Focal adhesion kinase 2
      • FADK 2
      • Proline-rich tyrosine kinase 2
      • Related adhesion focal tyrosine kinase
      • RAFTK
      References
      Product Information
      Quality SegmentMQ100
      Applications
      ApplicationActive, N-Terminal His6-tagged, recombinant, full-length, human Pyk2. For use in Kinase Assays.
      Key Applications
      • Kinase Assay
      Biological Information
      Sourceexpressed by baculovirus in Sf21 insect cells
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a cytoplasmic protein tyrosine kinase which is involved in calcium-induced regulation of ion channels and activation of the map kinase signaling pathway. The encoded protein may represent an important signaling intermediate between neuropeptide-activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. The encoded protein undergoes rapid tyrosine phosphorylation and activation in response to increases in the intracellular calcium concentration, nicotinic acetylcholine receptor activation, membrane depolarization, or protein kinase C activation. This protein has been shown to bind CRK-associated substrate, nephrocystin, GTPase regulator associated with FAK, and the SH2 domain of GRB2. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Four transcript variants encoding two different isoforms have been found for this gene.
      Gene Symbol
      • PTK2B
      • RAFTK
      • FAK2
      • PYK2
      • FADK2
      • PTK
      • CADTK
      • CAKB
      • PKB
      Protein TargetPyk2
      Purification MethodNi2+/NTA-agarose
      Target Sub-FamilyTK
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q14289 # Involved in calcium induced regulation of ion channel and activation of the map kinase signaling pathway. May represent an important signaling intermediate between neuropeptide activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. Interacts with the SH2 domain of Grb2. May phosphorylate the voltage-gated potassium channel protein Kv1.2. Its activation is highly correlated with the stimulation of c-Jun N-terminal kinase activity. Involved in osmotic stress-dependent SNCA 'Tyr-125' phosphorylation.
      SIZE: 1009 amino acids; 115875 Da
      SUBUNIT: Interacts with Crk-associated substrate (Cas), PTPNS1 and SH2D3C (By similarity). Interacts with nephrocystin, DDEF2, OPHN1L, SKAP2 and TGFB1I1.
      SUBCELLULAR LOCATION: Cytoplasm. Cell membrane. Note=Interaction with nephrocystin induces the membrane-association of the kinase.
      TISSUE SPECIFICITY: Most abundant in the brain, with highest levels in amygdala and hippocampus. Low levels in kidney. Also expressed in spleen and lymphocytes.
      PTM: Phosphorylated on tyrosine residues in response to various stimuli that elevate the intracellular calcium concentration, as well as by PKC activation. Recruitment by nephrocystin to cell matrix adhesions initiates Tyr-402 phosphorylation. In monocytes, adherence to substrata is required for tyrosine phosphorylation and kinase activation. Angiotensin II, thapsigargin and L-alpha- lysophosphatidic acid (LPA) also induce autophosphorylation and increase kinase activity (By similarity).
      SIMILARITY: SwissProt: Q14289 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily. & Contains 1 FERM domain. & Contains 1 protein kinase domain.
      Molecular Weight117.2kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by phosphorylation of Poly (Glu4-Tyr) (4:1)
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions6 months at -70°C
      Packaging Information
      Material Size10 µg
      Material PackageAlso available in 250 µg size --call for pricing and availability and reference catalog number 14-567M when ordering the 250 µg size.
      Transport Information
      Supplemental Information
      Specifications
      Global Trade ITEM Number
      Número de referencia GTIN
      14-567 04053252476082