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MABS2005 Anti-2A peptide Antibody, clone 3H4

Anti-2A peptide, clone 3H4, Cat. No. MABS2005, is a mouse monoclonal antibody that detects 2A peptide and has been tested in Immunofluorescence, Immunoprecipitation, and Western Blotting.

Anti-2A peptide Antibody, clone 3H4 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Secondary & Control Antibodies
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MABS2005
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MWB, IF, IPMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS2005
      DescriptionAnti-2A peptide Antibody, clone 3H4
      Background Information2A peptides and 2A-like peptide sequences (known as cis-acting hydrolase elements or CHYSEL) are a superior alternative to internal ribosomal entry sites (IRES) for coordinating the expression of multiple gene products from a single recombinant construct. The 2A sequences are relatively short peptides of about 20 amino acids (depending on the virus of origin) containing the consensus motif Asp-Val/Ile-Glu-X-Asn-Pro-Gly-Pro. 2A peptides allow multiple proteins to be encoded as polyproteins, which then dissociate into component proteins upon translation. The 2A sequence impairs normal peptide bond formation via a mechanism called ribosomal skipping, which results in effective, non-enzymatic generation of distinct peptide products from a single multicistronic construct. 2A peptides are used by several families of viruses, the best known foot-and-mouth disease virus of the Picornaviridae family, for producing multiple polypeptides. Anti-2A peptide, clone 3H4 recognizes 2A sequence derived from foot and mouth piconavirus (VKQTLNFDLLKLAGDVESNPG*P) with cleavage site between G and P. (Ref.: Trichas, G, et al. (2008). BMC Biol. 6: 40).
      References
      Product Information
      FormatPurified
      HS Code3002 15 90
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality SegmentMQ100
      Applications
      ApplicationAnti-2A peptide, clone 3H4, Cat. No. MABS2005, is a mouse monoclonal antibody that detects 2A peptide and has been tested in Immunofluorescence, Immunoprecipitation, and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunofluorescence
      • Immunoprecipitation
      Application NotesWestern Blotting Analysis: A representative lot detected 2A peptide in HEK293 SpCas9-P2A-Puro and NIH3T3 SpCas9-T2A-Puro cell lysates (Courtesy of Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria).

      Immunoprecipitation Analysis: A representative lot detected 2A peptide in HeLa cells expressing SpCas9-P2A or plain HeLa cells, HEK293 cells expressing SpCas9-T2A or plain HEK293 cells (Courtesy of Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria).

      Immunofluorescence Analysis: A representative lot detected 2A peptide in HeLa cells transiently transfected with SaCas9-T2A-GFP (Courtesy of Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria).
      Biological Information
      ImmunogenKLH-conjuated linear peptide corresponding to sequence CGDVEENPG (free C-term) derived from Thosea asigna virus.
      Clone3H4
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 3H4 detects 2A peptide in murine cells.
      IsotypeIgG1κ
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse.
      Antibody TypeMonoclonal Antibody
      Purification MethodProtein G purified
      Molecular Weight~150 kDa observed. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in NIH3T3 expressing SpCas9-T2A-Puro cell lysate.

      Western Blotting Analysis: 0.5 ug/mL of this antibody detected 2A peptide in 10 µg of NIH3T3 expressing SpCas9-T2A-Puro cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade ITEM Number
      Catalogue Number GTIN
      MABS2005 04054839250996

      Documentation

      Anti-2A peptide Antibody, clone 3H4 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-2A peptide Antibody, clone 3H4 Certificates of Analysis

      TitleLot Number
      Anti-2A peptide, clone 3H4 - 3192032 3192032
      Anti-2A peptide, clone 3H4 - 3197652 3197652
      Anti-2A peptide, clone 3H4 - 3379910 3379910
      Anti-2A peptide, clone 3H4 - 3745502 3745502
      Anti-2A peptide, clone 3H4 - 3799998 3799998
      Anti-2A peptide, clone 3H4 - 3843663 3843663
      Anti-2A peptide, clone 3H4 - 3875532 3875532
      Anti-2A peptide, clone 3H4 - 3880996 3880996
      Anti-2A peptide, clone 3H4 - 3902100 3902100
      Anti-2A peptide, clone 3H4 - 3991444 3991444
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