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MABT1354 Anti-CP110 Antibody, clone 140-195-5

Anti-CP110, clone 140-195-5 , Cat. No. MABT1354, is a mouse monoclonal antibody that detects Centriolar coiled-coil protein of 110 kDa and has been tested for use in immunocytochemistry, Immunofluorescence, Immunoprecipitation, and Western Blotting.

Anti-CP110 Antibody, clone 140-195-5 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Gene Symbol: CCP110, CEP110, CP110, KIAA0419 UniProt Number: O43303
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MABT1354
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HICC, IF, IP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABT1354
      DescriptionAnti-CP110 Antibody, clone 140-195-5
      Alternate Names
      • Centriolar coiled-coil protein of 110 kDa
      • Centrosomal protein of 110 kDa
      • Cep110
      Background InformationCentriolar coiled-coil protein of 110 kDa (UniProt: O43303; also known as Centrosomal protein of 110 kDa, CP110, Cep110) is encoded by the CCP110 (also known as CEP110, CP110, KIAA0419) gene (Gene ID: 9738) in human. CP110 is a cytoplasmic protein that is essential for centrosome duplication at different stages of procentriole formation. It acts as a key negative regulator of ciliogenesis in collaboration with CEP97 by capping the mother centriole thereby preventing cilia formation. It is also involved in the assembly of the mother centriole subdistal appendages thereby affecting the fusion of recycling endosomes to basal bodies during cilia formation. Two isoforms of CP110 have been reported that are produced by alternative splicing. CP110 is highly expressed in testis and intermediate level of expression is observed in spleen, thymus, prostate, small intestine, colon and peripheral blood leukocytes. CP110 levels re up-regulated during the transition from G1 to S phase of the cell cycle and highest levels are reported in S phase, following which the levels decline significantly. During the G2 phase it is ubiquitinated that leads to its proteasomal degradation, which prevents centrosome reduplication. And, de-ubiquitinated by USP33 in S and G2/M phase stabilizes CCP110 during the period when centrioles duplicate and elongate.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality SegmentMQ100
      Applications
      ApplicationAnti-CP110, clone 140-195-5 , Cat. No. MABT1354, is a mouse monoclonal antibody that detects Centriolar coiled-coil protein of 110 kDa and has been tested for use in immunocytochemistry, Immunofluorescence, Immunoprecipitation, and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Immunofluorescence
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation Analysis: A representative lot detected CP110 in HEK293T cell lysate (Courtesy of Prof. Erich A. Nigg, Biozentrum, University of Basel, Switzerland).

      Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected CP110 in U2OS cell line.

      Western Blotting Analysis: A representative lot detected CP110 in Western Blotting applications (Bauer, M., et. al. (2016). EMBO J. 35(19):2152-2166).

      Immunofluorescence Analysis: A representative lot detected CP110 in U20S cells (Courtesy of Prof. Erich A. Nigg, Biozentrum, University of Basel, Switzerland).

      Western Blotting Analysis: A representative lot detected CP110 in 293T cell lysate (Courtesy of Prof. Erich A. Nigg, Biozentrum, University of Basel, Switzerland).

      Immunofluorescence Analysis: A representative lot detected CP110 in Western Blotting applications (Bauer, M., et. al. (2016). EMBO J. 35(19):2152-2166; Arquint, C., et. al. (2014). Curr Biol. 24(4):351-60).
      Biological Information
      ImmunogenHis-tagged recombinant fragment corresponding to the first 149 amino acids from the N-terminal region of human Centriolar coiled-coil protein of 110 kDa.
      EpitopeN-terminus
      Clone140-195-5
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 140-195-5 detects Centriolar coiled-coil protein of 110 kDa (CP110) in human cells., It targets an epitope within the first 149 amino acids from the N-termina region.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CCP110
      • CEP110
      • CP110
      • KIAA0419
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~150 kDa observed; 113.42 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HEK293 cell lysate.

      Western Blotting Analysis: A 1:250 dilution of this antibody detected CP110 in HEK293 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade ITEM Number
      Catalogue Number GTIN
      MABT1354 04054839416613