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MABN2616-100UL Anti-TMEM30A Antibody, clone 7F4

Anti-TMEM30A, clone 7F4, Cat. No. MABN2616, is a mouse monoclonal antibody that detects TMEM30A and is tested for use in Immunohistochemistry, Immunoaffinity Purification, Immunoprecipitation, and Western Blotting.

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MABN2616-100UL
100 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABN2616-100UL
      DescriptionAnti-TMEM30A Antibody, clone 7F4
      Alternate Names
      • Cell cycle control protein 50A
      • CDC50A
      • P4-ATPase flippase complex beta subunit
      • Transmembrane protein 30A
      Background InformationCell cycle control protein 50A (UniProt: Q17QL5; also known as P4-ATPase flippase complex beta subunit TMEM30A, Transmembrane protein 30A) is encoded by the TMEM30A gene (Gene ID: 505797) in bovine species. TMEM30A is a multi-pass membrane protein with two cytoplasmic domains, two transmembrane domains, and one extracellular domain. It is expressed in photoreceptor cells and is detected in retina outer segment and other retinal layers. It is a component of various P4-ATPase flippase complexes, which consists of a catalytic -subunit and an accessory β-subunit. It catalyzes the hydrolysis of ATP coupled to the transport of aminophospholipids from the outer to the inner leaflet of various membranes and ensures the maintenance of asymmetric distribution of phospholipids. The β-subunit may assist in binding of the phospholipid substrate. Most P4-ATPases associate with one of three accessory subunit isoforms known as CDC50A (TMEM30A), CDC50B (TMEM30B), and CDC50C (TMEM30C). TMEM30A is required for the proper folding, assembly and endoplasmic reticulum (ER) to Golgi exit of the ATP8A2:TMEM30A flippase complex. This ATP8A2:TMEM30A complex may be involved in regulation of neurite outgrowth. It is also involved in the uptake of platelet-activating factor (PAF).
      (Ref.: Liou, AY., et al. (2019). J. Biol. Chem. 294(17); 6809-6821; Wang, J., et al. (2018). Sci. Rep. 8: 10795).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality SegmentMQ200
      Applications
      ApplicationAnti-TMEM30A, clone 7F4, Cat. No. MABN2616, is a mouse monoclonal antibody that detects TMEM30A and is tested for use in Immunohistochemistry, Immunoaffinity Purification, Immunoprecipitation, and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • Immunoaffinity Purification
      • Immunoprecipitation
      Application NotesTested applications

      Western Blotting Analysis: A 1:500 dilution from a representative lot detected TMEM30A in Human brain, mouse brain, and mouse liver tissue lysates.

      Immunoaffinity Purification: A representative lot was used in Immunoaffinity Purification of TMEM30A. (Wang, J., et al. (2018). Sci Rep. 8(1):10795).

      Immunohistochemistry Applications: A representative lot detected TMEM30A in Immunohistochemistry applications (Coleman, J.A., et al. (2011). J Biol Chem. 286(19):17205-16).

      Western Blotting Analysis: A representative lot detected TMEM30A in Western Blotting applications (Coleman, J.A., et al. (2011). J Biol Chem. 286(19):17205-16; Wang, J., et al. (2018). Sci Rep. 8(1):10795; Liou, A.Y., et al. (2019). J Biol Chem. 294(17):6809-6821).

      Immunoprecipitation Analysis: A representative lot detected TMEM30A in Immunoprecipitation applications (Coleman, J.A., et al. (2011). J Biol Chem. 286(19):17205-16).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenA linear peptide corresponging to 9 amino acids from the N-terminal region of bovine cell cycle control protein 50A (TMEM30A).
      EpitopeN-terminal
      Clone7F4
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 7F4 is a mouse monoclonal antibody that detects Cell cycle control protein 50A (TMEM30A). It targets an epitope within 9 amino acids from the N-terminal, cytoplasmic domain.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse. Predicted to react with Porcine, Feline, Bovine, Canine, Monkey, Horse based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • TMEM30A
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight52-54 kDa observed; 40.68 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Mouse testis tissue lysate.

      Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected TMEM30A in Mouse testis tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade ITEM Number
      Catalogue Number GTIN
      MABN2616-100UL 04065268900796