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Merck

14420C

EX-CELL® 420 Serum-Free Medium for Insect Cells

with L-glutamine, sterile-filtered, suitable for insect cell culture

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UNSPSC Code:
12352207
NACRES:
NA.75
MDL number:
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description

for research or for further manufacturing use

Quality Segment

sterility

sterile-filtered

product line

EX-CELL®

form

liquid

technique(s)

cell culture | insect: suitable

components

HEPES: no
L-glutamine: yes
glucose: yes
NaHCO3: yes

shipped in

ambient

storage temp.

2-8°C

Application

EX-CELL 420 is a complete medium developed and optimized for the serum-free growth of Sf9 and Sf21 insect cell lines. Cells can be subcultured directly into EX-CELL 420 from serum-free or serum-supplemented media without adaptation. Cultures in EX-CELL 420 routinely reach cell densities greater than 1 × 107 cells/mL with greater than 95% viability. Suspension cultures can be maintained, without refeeding, for more than 10 days. Sf9 and Sf21 cells have been carried for more than 20 passages in EX-CELL 420 with no loss of viability. Protein expression and virus production are improved over serum-containing media.
EX-CELL 420 is a complete medium developed and optimized for the serum-free growth of Sf9 and Sf21 insect cell lines. Cells can be subcultured directly into EX-CELL 420 from serum-free or seum-supplemented media without adaptation. Cultures in EX-CELL 420 routinely reach cell densities greater than 1 × 107 cells/mL with greater than 95% viability. Suspension cultures can be maintained, without refeeding, for more than 10 days. Sf9 and Sf21 cells have been carried for more than 20 passages in EX-CELL 420 with no loss of viability. Protein expression and virus production are improved over serum-containing media.

Legal Information

EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany


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10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable



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