MAB318
Anti-Tyrosine Hydroxylase Antibody
CHEMICON®, mouse monoclonal, LNC1
Synonym(s):
TH, Tyrosine Monooxygenase, Tyrosine 3-monooxygenase
Sign In to View Organizational & Contract Pricing.
Select a Size
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Product Name
Anti-Tyrosine Hydroxylase Antibody, clone LNC1, ascites fluid, clone LNC1, Chemicon®
biological source
mouse
antibody form
ascites fluid
antibody product type
primary antibodies
clone
LNC1, monoclonal
species reactivity
zebrafish, mouse, rat, vole, human, frog, lizard, monkey, chicken
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Analysis Note
Control
Human brain tissue, extracts from 3T3 cells treated with Forskolin (40 nM, 30 min).
Human brain tissue, extracts from 3T3 cells treated with Forskolin (40 nM, 30 min).
Routinely evaluated by Western Blot on Mouse Brain lysates.
Western blot:
1:1000 dilution of this lot detected Tyrosine Hydroxylase on 10 μg of Mouse Brain lysates.
Western blot:
1:1000 dilution of this lot detected Tyrosine Hydroxylase on 10 μg of Mouse Brain lysates.
Application
Detect Tyrosine Hydroxylase using this Anti-Tyrosine Hydroxylase Antibody, clone LNC1 validated for use in IH, IH(P), IP & WB with more than 85 product citations.
Immunohistochemistry(paraffin):
A 1:200-1:400 dilution of a previous lot was used in IH. 4% PFA fixed, frozen sections; 4% PFA fixed, paraffin sections 1:100 (Barrachina, M. et al., 2003). For paraffin sections Barrachina reported successful staining with microwave citrate acid antigen recovery, however other methods can likely be used as well.
Immunoprecipitation:
A previous lot of this antibody was used in IP (Perez, 2002).
Optimal working dilutions and protocols must be determined by end user.
A 1:200-1:400 dilution of a previous lot was used in IH. 4% PFA fixed, frozen sections; 4% PFA fixed, paraffin sections 1:100 (Barrachina, M. et al., 2003). For paraffin sections Barrachina reported successful staining with microwave citrate acid antigen recovery, however other methods can likely be used as well.
Immunoprecipitation:
A previous lot of this antibody was used in IP (Perez, 2002).
Optimal working dilutions and protocols must be determined by end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Neuronal & Glial Markers
Neurotransmitters & Receptors
Neuronal & Glial Markers
Biochem/physiol Actions
Recognizes an epitope on the outside of the regulatory N-terminus. Recognizes a protein of approximately 59-61 kDa by Western blot. Does not react with the following on Western Blots: dopamine-beta-hydroxylase, phenylalanine hydroxylase, trytophan hydroxylase, dehydropteridine reductase, sepiapterin reductase or phenethanolamine-N-methyl transferase (PNMT).
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
59-63 kDa
Tyrosine hydroxylase plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of the catecholamines Dopamine and Norepinephrine from tyrosine. TH is, therefore, a useful marker for dopaminergic and noradrenergic neurons. The enzymatic activity of TH requires ferrous ions as cofactors and is believed to be regulated by phosphorylation. At least four isoforms of human TH have been identified which result from alternative splicing.
Immunogen
Tyrosine Hydroxylase purified from PC12 cells
Physical form
Ascites mouse monoclonal IgG1κ fluid containing PBS pH 7.1 , contains no preservative.
Unpurified
Preparation Note
Stable for 1 year at -20ºC from date of receipt.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Not finding the right product?
Try our Product Selector Tool.
Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Suppression of prolactin-induced signal transducer and activator of transcription 5b signaling and induction of suppressors of cytokine signaling messenger ribonucleic acid in the hypothalamic arcuate nucleus of the rat during late pregnancy and lactation.
Anderson, GM; Beijer, P; Bang, AS; Fenwick, MA; Bunn, SJ; Grattan, DR
Endocrinology null
A FRET-based method to study protein thiol oxidation in histological preparations.
Mastroberardino, PG; Orr, AL; Hu, X; Na, HM; Greenamyre, JT
Free radical biology & medicine null
Expression patterns of the opsin 5-related genes in the developing chicken retina.
Sayuri Tomonari,Kyoichi Migita,Akira Takagi,Sumihare Noji,Hideyo Ohuchi
Developmental Dynamics null
Age and region-specific responses of microglia, but not astrocytes, suggest a role in selective vulnerability of dopamine neurons after 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine exposure in monkeys.
Kanaan, NM; Kordower, JH; Collier, TJ
Glia null
Paraskevi Krashia et al.
Nature communications, 10(1), 4725-4725 (2019-10-16)
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
Global Trade Item Number
| SKU | GTIN |
|---|---|
| MAB318 | 08436037123320 |
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service