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Merck

MAB1281

Anti-Nuclei Antibody, clone 235-1

clone 235-1, Chemicon®, from mouse

Synonym(s):

HuNu

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

Key Documents

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Product Name

Anti-Nuclei Antibody, clone 235-1, clone 235-1, Chemicon®, from mouse

biological source

mouse

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

235-1, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable

input

sample type neural stem cell(s)

isotype

IgG1

shipped in

dry ice

target post-translational modification

unmodified

Quality Level

100

Analysis Note

Control
All human cell types

Application

Anti-Nuclei Antibody, clone 235-1 has been used in: Immunohistochemistry (1:200); Immunolabeling for human nuclei (HuNu) (1:300); Histological and immuno-fluorescent analysis of submandibular glands

Biochem/physiol Actions

Stains nuclei of all human cell types giving a diffuse nuclear staining pattern. Chromosomes are negatively stained in metaphase cells. Also stains nuclei from primate (Sanchez-Pernaute, 2005). The antibody does not react with nuclei from mouse or rat.
The human nuclei monoclonal reacts specifically with human cells. It shows no reactivity against mouse, rat, chicken in immunohistorchemistry. Other mammalian or animal species have not been tested for reactivity.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Cell replacement techniques such as stem-cell or fetal-cell therapies show great promise for many diseases. Cross-species grafting between human and animal cells or tissues requires the identification and localization of the donor material in the host environment. Anti-nuclei antibodies are useful cytological and histological markers for identifying human cells in xenograft models.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Physical form

Format: Purified
Hybridoma supernatant. Liquid in PBS. Contains no preservative.
Protein A purified

Preparation Note

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Long-term hibernation of human fetal striatal tissue does not adversely affect its differentiation in vitro or graft survival: implications for clinical trials in Huntington's disease.
Hurelbrink, Carrie B, et al.
Cell Transplantation, 12, 687-695 (2003)
Improving the survival of human CNS precursor-derived neurons after transplantation.
Le Belle, J E, et al.
Journal of Neuroscience Research, 76, 174-183 (2004)
Infusion of human umbilical cord blood cells in a rat model of stroke dose-dependently rescues behavioral deficits and reduces infarct volume.
Vendrame, Martina, et al.
Stroke, 35, 2390-2395 (2004)
Transplantation of cultured neural cells from human fetuses into the brain of rats exposed to acute hypoxia.
Aleksandrova, M A, et al.
Bulletin of experimental biology and medicine, 137, 262-265 (2004)
Human umbilical cord blood cells express neural antigens after transplantation into the developing rat brain.
Zigova, Tanja, et al.
Cell Transplantation, 11, 265-274 (2002)
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Global Trade Item Number

SKUGTIN
MAB128104053252317378

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