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About This Item
material
colorless polypropylene , colorless wells
sterility
non-sterile
feature
plate format: 96 well standard
packaging
case of 25 ea
manufacturer/tradename
Sorenson 2633
technique(s)
PCR: suitable
well maximum volume
300 μm
working volume
200 μL
suitability
suitable for (PCR, RT-PCR or DNA purification applications)
size
96 wells
application(s)
agriculture
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General description
Features and Benefits
- Virgin polypropylene
- Fully autoclavable
- Certified DNase- and RNase-free
- Wells have thin walls for rapid temperature equilibration and reduced cycle time
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Protocols
使用抗体介导热启动聚合酶的实验方案。一种具有短活化阶段的方法(<1min), and results in higher yields and more specificity over standard PCR methods.
使用逆转录酶和dNTPs进行逆转录(RT)使用总RNA或基因特异性方法实现仅目标RNA被转化为cDNA。
了解标准 PCR 方案步骤,查看试剂清单或循环参数。本方法使用标准 Taq DNA 聚合酶对 DNA 进行常规 PCR 扩增。
逆转录是一种旨在测量基因mrna浓度的基因表达分析。 这种分析存在多个挑战,例如不同转录本之间的半衰期差异、时间模式和缺乏相关蛋白。
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