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Merck

Z374903

PCR multiwell plates

96 well plate for PCR

Synonym(s):

96 mulitwell PCR plate, 96 well PCR microplate, 96 well PCR plate, 96 well microtiter plate

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About This Item

NACRES:
NB.22
UNSPSC Code:
41106300
Material:
colorless polypropylene , colorless wells
Size:
96 wells
Sterility:
non-sterile
Feature:
plate format: 96 well standard
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material

colorless polypropylene , colorless wells

sterility

non-sterile

feature

plate format: 96 well standard

packaging

case of 25 ea

manufacturer/tradename

Sorenson 2633

technique(s)

PCR: suitable

well maximum volume

300 μm

working volume

200 μL

suitability

suitable for (PCR, RT-PCR or DNA purification applications)

size

96 wells

application(s)

agriculture

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General description

A rigid top plate (included) minimizes plate distortion, assures a dependable fit with the PCR thermal cycler, and allows for a leak-proof seal with micro-mats or cap strips. Each well has a maximum capacity of 200 μL.

Features and Benefits

  • Virgin polypropylene
  • Fully autoclavable
  • Certified DNase- and RNase-free
  • Wells have thin walls for rapid temperature equilibration and reduced cycle time

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Protocols

使用抗体介导热启动聚合酶的实验方案。一种具有短活化阶段的方法(<1min), and results in higher yields and more specificity over standard PCR methods.

使用逆转录酶和dNTPs进行逆转录(RT)使用总RNA或基因特异性方法实现仅目标RNA被转化为cDNA。

了解标准 PCR 方案步骤,查看试剂清单或循环参数。本方法使用标准 Taq DNA 聚合酶对 DNA 进行常规 PCR 扩增。

逆转录是一种旨在测量基因mrna浓度的基因表达分析。 这种分析存在多个挑战,例如不同转录本之间的半衰期差异、时间模式和缺乏相关蛋白。

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