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03-0188-00

SMC® Human Phospho-α-Synuclein (S129) High Sensitivity Immunoassay Kit

Used for ultrasensitive quantification of Human Phospho-α-Synuclein (S129) in human serum, plasma, and cerebrospinal fluid (CSF)

Synonym(s):

p-α-synuclein S129 immunoassay kit, p-α-synuclein Ser129 immunoassay kit, p-α-synuclein Single Molecule Counting Kit, p-α-synuclein ultra-sensitive immunoassay

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About This Item

NACRES:
NA.84
UNSPSC Code:
12161503
Species reactivity:
human
Technique(s):
single molecule counting: suitable
Usage:
 kit sufficient for 96 wells
Compatibility:
for use with FemtoQuest™ and SMCxPRO® instruments
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Product Name

SMC® Human Phospho-α-Synuclein (S129) High Sensitivity Immunoassay Kit,

description

Used for ultrasensitive quantification of Human Phospho-α-Synuclein (S129) in human serum, plasma, and cerebrospinal fluid (CSF)

product line

SMC®

usage

 kit sufficient for 96 wells

species reactivity

human

technique(s)

single molecule counting: suitable

input

cerebrospinal fluid (CSF)
serum
plasma (K2 EDTA)

UniProt accession no.

P37840

compatibility

for use with FemtoQuest™ and SMCxPRO® instruments

shipped in

wet ice

storage temp.

2-8°C

General description

Phospho-α-synuclein (S129) is a modified form of the protein α-synuclein, characterized by the phosphorylation at serine 129. This post-translational modification influences the protein′s structure and function, promoting its aggregation into toxic fibrils, which are a hallmark of several neurological disorders. In healthy neurons, α-synuclein plays a role in synaptic function, regulating neurotransmitter release and maintaining synaptic integrity. However, when phosphorylated at S129, α-synuclein tends to misfold and aggregate, leading to the formation of Lewy bodies, which are implicated in neurodegenerative diseases such as Parkinson′s disease and dementia with Lewy bodies.

Phospho-α-synuclein (S129) also contributes to neuroinflammation by activating microglial cells, which can exacerbate neuronal damage and promote disease progression. Its presence in the brain serves as a biomarker for neurodegeneration, highlighting its critical role in the pathophysiology of various neurodegenerative conditions.

Application

Neurological DisordersNeurodegenerative DiseasesSynucleinopathies

Features and Benefits

  • Femtogram-Scale Analysis: Achieves ultra-low detection limits (fg/mL) in serum, plasma or CSF.
  • Advanced Signal Optimization: Incorporates expanded dynamic range with reduced background noise, ensuring reproducibility.
  • Seamless Workflow: Combines a familiar sandwich immunoassay architecture with next-generation sensitivity.
  • Automation Compatibility: Supports robotic liquid handling integration to reduce touch time and improve efficiency.
  • Technical Expertise: Supported by skilled field scientists and technical teams with SMC® expertise.

Packaging

96 well assay

Analysis Note

  • Sensitivity: LLOQ 100 fg/mL* (SMCxPRO® Instrument)
  • Standard Curve Range: 50-50,000 fg/mL
  • Intra-assay CV: <15%
  • Inter-assay CV: <20%
  • Linearity: Serum: 101% Plasma: 96% CSF: 99%
  • Recovery: Serum: 85% Plasma: 89% CSF: 100%
*Indicative LLOQ created from average of 14 independent in house runs, where >80% met same value defined with CV & Recovery +/- 20%. Refer to CoA for lot specific performance.

Other Notes

Although Phospho-α-Synuclein (S129) was detectable in non-human primate (rhesus macaque and cynomolgus monkey), feline, rat, mouse, and canine samples, the assay has not been verified for these species.

Legal Information

FEMTOQUEST is a trademark of Merck KGaA, Darmstadt, Germany
SMC is a registered trademark of Singulex
SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

pictograms

Skull and crossbonesHealth hazard
GHS06,GHS08

signalword

Danger

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - ED ENV 1 - Eye Irrit. 2 - Repr. 1B

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