Anti-CSPG4 Antibody

270-300 kDa

Chondroitin sulfate proteoglycan 4 (UniProt: Q00657; also known as Chondroitin sulfate proteoglycan NG2, HSN tumor-specific antigen) is encoded by the Cspg4 (also known as Ng2) gene (Gene ID: 81651) in rat. Chondroitin sulfate proteoglycan NG2 is a single-pass type I membrane protein that plays a role in cell proliferation and migration and stimulates endothelial cells motility during microvascular morphogenesis. It is present in neural cells and in extraneural tissues, especially in the developing mesenchyme. Its level of expression is highest on immature, proliferating cells and decreases when these cells begin to differentiate. It is found on the surfaces of an unusual class of glial cells within the developing and mature central nervous system that have the properties of oligodendrocyte precursor cells. It is a major chondroitin sulfate proteoglycan that is produced after spinal cord injury and is expressed by macrophages and oligodendrocyte progenitors. It is also reported to inhibit neurite outgrowth and growth cone collapse during axon regeneration. NG2 chondroitin sulfate proteoglycan is synthesized with a signal peptide (aa 1-29), which is subsequently cleaved off in the mature form.(Ref.: Jones, LL et al. (2002). J. Neurosci. 22(7):2792-2803).

Immunoaffinity purified NG2 Chondroitin Sulfate Proteoglycan from rat.

Detect NG2 Chondroitin Sulfate Proteoglycan using this Anti-NG2 Chondroitin Sulfate Proteoglycan Antibody validated for use in ELISA, IC, IH, IP and WB.

Immunocytochemistry:
A 1:150-1:600 dilution of a previous lot was used in IC.

Immunohistochemistry:
1:200 dilution of a previous lot was used on frozen sections of embryonic mouse brain using an Alexa Fluor conjugated secondary antibody. Care should be taken to avoid overfixing tissue sections. A short (5-10 minute) fix in 2-4% PFA is recommended.

Immunoprecipitation:
2 μg/mL concentration of a previous lot was used in IP.

ELISA:
A 1:1,500-1:3,000 dilution of a previous lot was used in ELISA.

Optimal working dilutions and protocols must be determined by end user

Research Category
Neuroscience

Research Sub Category
Neuronal & Glial Markers

NG2 Chondroitin Sulfate Proteoglycan. AB5320 identifies both the intact proteoglycan and the core protein by Western blot and ELISA. When oligodendrocyte precursor cells (i.e. O-2A progenitor cells) are stained alive, the stain appears as clusters on the cell surface. This antibody does not stain differentiated oligodendrocytes well.

Antibody purified by protein A, then placed over a fibronectin affinity column.

Purified rabbit polyclonal in PBS buffer containing 0.02% azide.

Stable for 6 months at -20ºC in undiluted aliquots from date of receipt.

Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Control
Human melanoma, glioma and proliferating brain endothelial cells, rat B49 glial cell extracts or embryonic mouse brain tissue.

Routinely evaluated by Western Blot on rat brain lysates

Western Blot: 1:1000 dilution of this lot detected NG2 chondroitin sulfate on 10 μg of rat brain lysates.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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