B6024
5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside
tablet, suitable for identification of lac+ bacterial colonies
Synonym(s):
5-Bromo-4-chloro-3-indolyl β-D-galactoside, BCIG, X-Gal
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About This Item
Empirical Formula (Hill Notation):
C14H15BrClNO6
CAS Number:
Molecular Weight:
408.63
NACRES:
NA.83
PubChem Substance ID:
UNSPSC Code:
12352204
EC Number:
230-640-8
MDL number:
Beilstein/REAXYS Number:
1402009
Product Name
5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside, tablet
form
tablet
suitability
suitable for identification of lac+ bacterial colonies
storage temp.
−20°C
SMILES string
OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)c(Cl)c23)[C@H](O)[C@@H](O)[C@H]1O
InChI
1S/C14H15BrClNO6/c15-5-1-2-6-9(10(5)16)7(3-17-6)22-14-13(21)12(20)11(19)8(4-18)23-14/h1-3,8,11-14,17-21H,4H2/t8-,11+,12+,13-,14-/m1/s1
InChI key
OPIFSICVWOWJMJ-AEOCFKNESA-N
General description
5-Bromo-4-chloro-3-indolyl β -D-galactopyranoside, commonly known as X-Gal, is a histochemical substrate for β-galactosidase.
Application
X-Gal is a chromogenic substrate for β-galactosidase that produces a rich blue color that can easily be detected visually over background. X-Gal is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype.
5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside has been used in the preparation of X-Gal stock solution to add it in LB agar plates for blue-white screening of transformants.
Biochem/physiol Actions
X-gal is routinely used to detect transgene activity in situ.
Preparation Note
A stock solution (100 mg/ml) is prepared by dissolving X-Gal in dimethylformamide and should be stored at −20°C and protected from light. Sterilization is not required.
Contains 5 mg substrate per tablet
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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