C8302
4-Chloro-1-naphthol solution
For HRP detection on Western Blots
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About This Item
CAS Number:
NACRES:
NA.52
PubChem Substance ID:
UNSPSC Code:
12352200
MDL number:
grade
Molecular Biology
Quality Segment
form
liquid
storage temp.
2-8°C
SMILES string
Oc1ccc(Cl)c2ccccc12
InChI
1S/C10H7ClO/c11-9-5-6-10(12)8-4-2-1-3-7(8)9/h1-6,12H
InChI key
LVSPDZAGCBEQAV-UHFFFAOYSA-N
General description
4-Chloro-1-naphthol is a substrate for HRP in a reaction that results in a colored precipitate. It is commonly used for colorimetric detection and visualization of proteins in western blotting.
Application
Suitable for visualizing protein-peroxidase conjugates in western blotting.
Biochem/physiol Actions
Enzymes such as HRP convert 4-Chloro-1-naphthol to a purple-colored precipitate on the blot that is readily visible to the eye.
Other Notes
0.48 mM 4-chloro-1-naphthol, 50 mM Tris-HCl, 0.2 M NaCl and 17% methanol.
This product contains 0.48 mM 4-chloro-1-naphthol, 50 mM Tris-HCl, 0.2 M NaCl and 17% methanol.
signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - STOT SE 1
target_organs
Eyes,Central nervous system
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 2
flash_point_f
163.9 °F - closed cup
flash_point_c
73.3 °C - closed cup
ppe
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
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