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F4523

Fast Red TR/Naphthol AS-MX

SIGMAFAST™, fluorogenic, tablet

Synonym(s):

4-Chloro-2-methylbenzenediazonium / 3-Hydroxy-2-naphthoic acid 2,4-dimethylanilide phosphate tablets

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.83
MDL number:
MFCD00132253
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Product Name

SIGMAFAST™ Fast Red TR/Naphthol AS-MX Tablets, tablet, To prepare 10 mL

Quality Level

200

form

tablet

storage temp.

−20°C

Application

SIGMAFAST™ Fast Red TR/Naphthol AS-MX tablets has been used in fluorescent in situ hybridization for the analysis of gene expression in neurons and synapses.
Developed for use as a precipitating substrate for the detection of alkaline phosphatase activity in immunochemistry. Primary uses are in immunohistology, immunoblotting, or dot blotting. SIGMAFAST™ Fast Red TR/Naphthol AS-MX tablets contain 0.6 mM levamisole to block endogenous alkaline phosphatase activity. SIGMAFAST™ Fast Red TR/Naphthol AS-MX Tablets require no additional steps or buffers to prepare an active substrate solution.

Preparation Note

Each tablet set dissolved in 10 mL of deionized water yields a ready-to-use buffered solution.

Legal Information

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

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Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Sol. 2 - Skin Irrit. 2

Storage Class

4.1B - Flammable solid hazardous materials

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Articles

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

比色碱性磷酸酶和过氧化物酶底物检测系统

硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。

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