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Merck

W1754

Water

PCR Reagent, suitable for PCR

Synonym(s):

H2O

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About This Item

Linear Formula:
H2O
CAS Number:
7732-18-5
Molecular Weight:
18.02
NACRES:
NA.25
PubChem Substance ID:
24900779
UNSPSC Code:
12191602
EC Number:
231-791-2
MDL number:
MFCD00011332
Beilstein/REAXYS Number:
2050024
Technique(s):
PCR: suitable
Bp:
100 °C (lit.)
Vapor pressure:
3 mmHg

Key Documents

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Product Name

Water, PCR Reagent

InChI key

XLYOFNOQVPJJNP-UHFFFAOYSA-N

InChI

1S/H2O/h1H2

vapor density

<1 (vs air)

vapor pressure

3 mmHg

sterility

sterile-filtered

Quality Level

200

packaging

vial of 1.5 mL

technique(s)

PCR: suitable

pH

5-7

bp

100 °C (lit.)

mp

0 °C (lit.)

foreign activity

DNase, none detected, RNase, none detected

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General description

PCR grade water is sterile-filtered. It is free from exonucleases (DNAse, RNAse) and endonuclease (NICKase) and is also free from nucleic acid contamination.

Application

Suitable for polymerase chain reaction (PCR)
Water has been used:

  • as a component of the reaction mixture and as a diluting agent in microfluidic RT-qPCR
  • as a component of the reaction mixture for the amplification of products from fungal (Trametes versicolor) DNA
  • as a diluting agent and as a component of the reaction mixture for the amplification of cDNA
  • Water has been used to make up the final volume of the sample in polymerase chain reaction (PCR)

Other Notes

Easily compare specifications for Water products with the Water specification table.

Storage Class

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
SLCJ6000
SLCH8408
SLCH7893
SLCH2893
SLCG4584

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J Loeffler et al.
Journal of clinical microbiology, 37(4), 1200-1202 (1999-03-13)
Successful in vitro amplification of fungal DNA in clinical specimens has been reported recently. In a collaboration among five European centers, the frequency and risk of contamination due to airborne spore inoculation or carryover contamination in fungal PCR were analyzed.
Francine Marciano-Cabral et al.
Applied and environmental microbiology, 69(10), 5864-5869 (2003-10-09)
The free-living amoeboflagellate Naegleria fowleri is the causative agent of primary amoebic meningoencephalitis (PAM), a rapidly fatal disease of the central nervous system. In the United States, the disease is generally acquired while swimming and diving in freshwater lakes and
If you?ve seen one worm, have you seen them all? Spatial, community, and genetic variability of tubificid communities in Montana
Lodh N, et al.
Freshwater science, 34(3), 909-917 (2015)
Multiplex PCR for rapid detection of genes encoding acquired metallo-beta-lactamases.
Matthew J Ellington et al.
The Journal of antimicrobial chemotherapy, 59(2), 321-322 (2006-12-23)
Incidence and survival of non-O157 verocytotoxigenic Escherichia coli in soil
Bolton DJ, et al.
Journal of Applied Microbiology, 111(2), 484-490 (2011)
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标准 PCR 程序

了解标准 PCR 方案步骤，查看试剂清单或循环参数。本方法使用标准 Taq DNA 聚合酶对 DNA 进行常规 PCR 扩增。

标准逆转录方案（两步法）

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