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Merck

W4502

Water

suitable for electrophoresis, PCR

Synonym(s):

DNase-Free Water, Not DEPC Treated, Nuclease-Free Water, Protease-Free Water, RNase-Free Water

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About This Item

Linear Formula:
H2O
CAS Number:
Molecular Weight:
18.02
NACRES:
NA.31
PubChem Substance ID:
UNSPSC Code:
12191602
EC Number:
231-791-2
MDL number:
Beilstein/REAXYS Number:
2050024
Technique(s):
PCR: suitable, electrophoresis: suitable
Bp:
100 °C (lit.)
Vapor pressure:
3 mmHg
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Product Name

Water, Nuclease-Free Water, for Molecular Biology

InChI key

XLYOFNOQVPJJNP-UHFFFAOYSA-N

InChI

1S/H2O/h1H2

vapor density

<1 (vs air)

vapor pressure

3 mmHg

sterility

0.1 μm filtered

Quality Level

technique(s)

PCR: suitable, electrophoresis: suitable

pH

5-7

bp

100 °C (lit.)

mp

0 °C (lit.)

foreign activity

DNase, RNase, Protease, free

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Application

Water has been used-
  • in cDNA synthesis and PCR amplification
  • for the dilution of rumen DNA sample prior to qPCR assay
  • in PCR (polymerase chain reaction) and agarose gel electrophoresis
  • for the elution of RNA during RNA isolation

Features and Benefits

  • Nuclease-Free Water
  • DNase-Free, RNase-Free, Protease- Free
  • Not DEPC Treated
  • 0.1 um Filtered
  • Prepared from Double Distilled and Deionized Water
  • Suitable for use in Molecular Applications

This product is 0.1um filtered. It is designated as a Molecular Biology Reagent and is suitable for molecular biology applications. This product has been analyzed for the absence of nucleases and proteases, and has undergone bioburden analysis. It has not been treated with diethyl pyrocarbonate (DEPC).

Preparation Note

This product is prepared from double distilled and deionized water

Other Notes

Easily compare specifications for Water products with the Water specification table.

Storage Class

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves


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Comparison of coliforms and coliphages as tools for assessment of viral contamination in river water.
Skraber S et al
Applied and Environmental Microbiology, 70(6), 3644-3649 (2004)
Presence of viral genomes in mineral water: a sufficient condition to assume infectious risk?
Gassilloud B et al
Applied and Environmental Microbiology, 69(7), 3965-3969 (2003)
Polymorphic exact tandem repeat A (PETRA): a newly defined lineage of mycobacterium tuberculosis in israel originating predominantly in Sub-Saharan Africa.
Freidlin PJ et al
Journal of Clinical Microbiology, 47(12), 4006-4020 (2009)
Effect of sward dry matter digestibility on methane production, ruminal fermentation, and microbial populations of zero-grazed beef cattle.
Hart KJ et al
Journal of Animal Science, 87(10), 3342-3350 (2009)
Gene expression signals involved in ischemic injury, extracellular matrix composition and fibrosis defined by global mRNA profiling of the human left ventricular myocardium.
Gabrielsen A et al
Journal of Molecular and Cellular Cardiology, 42(4), 870-883 (2007)

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