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Merck

Z374873

PCR microtubes with attached caps

capacity 0.2 mL

Synonym(s):

PCR Tubes

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About This Item

NACRES:
NB.22
UNSPSC Code:
41121702
Capacity:
0.2 mL
Material:
colorless cap, colorless tube, conical bottom, flat cap, polypropylene , polypropylene cap, polypropylene tube
Packaging:
pack of 250 ea, case of 4 packs
Feature:
RNase and DNase free, autoclavable at 121 °C, graduations: no, Push fit

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material

colorless cap, colorless tube, conical bottom, flat cap, polypropylene , polypropylene cap, polypropylene tube

description

Autoclavable

feature

RNase and DNase free, autoclavable at 121 °C, graduations: no, Push fit

packaging

pack of 250 ea, case of 4 packs

technique(s)

PCR: suitable

capacity

0.2 mL

color

clear

suitability

suitable for (PCR applications)

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General description

Thin-walled for efficient thermal transfer and shorter cycle times; fits all leading thermal cyclers including PerkinElmer, Biometra, MJ Research, Techne, Grant and Strategene (0.65 mL only). Certified DNase- and RNase-free. All tolerate organic solvent reactions and temperatures from -4 to 121 °C.

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Protocols

终点 PCR：特异性和产量增强的抗体介导的热启动 PCR 方案

使用抗体介导热启动聚合酶的实验方案。一种具有短活化阶段的方法（<1min), and results in higher yields and more specificity over standard PCR methods.

使用SYBR Green染料检测的逆转录实验方案

使用逆转录酶和dNTPs进行逆转录（RT）使用总RNA或基因特异性方法实现仅目标RNA被转化为cDNA。

标准 PCR 程序

了解标准 PCR 方案步骤，查看试剂清单或循环参数。本方法使用标准 Taq DNA 聚合酶对 DNA 进行常规 PCR 扩增。

标准逆转录方案（两步法）

逆转录是一种旨在测量基因mrna浓度的基因表达分析。 这种分析存在多个挑战，例如不同转录本之间的半衰期差异、时间模式和缺乏相关蛋白。

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