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About This Item
NACRES:
NB.22
UNSPSC Code:
41122410
Manufacturer/tradename:
Excel Scientific TS-RT2RR-100
Material:
polyester film
W × l:
7.78 cm × 13.08 cm
Sterility:
non-sterile
Packaging:
pack of 100 ea
material
polyester film
sterility
non-sterile
packaging
pack of 100 ea
manufacturer/tradename
Excel Scientific TS-RT2RR-100
technique(s)
PCR: suitable, qPCR: suitable
L
130.6 mm
W × L
7.78 cm × 13.08 cm
W
77.8 mm
color
clear
General description
A combination of optically transparent polyester film with a strong, ultra-smooth, non-absorbing, non-fluorescing medical-grade adhesive for superior performance in real-time PCR applications. In place of the customary paper backing, ThermalSeal RT has a plastic liner which is easily removed before use and contributes smoothness and extreme optical clarity to the adhesive. Dimensions are 3.125 x 5.625 in. to seal PCR plates. Length between the perforated ends is 4.8 in.
- Ultra-high optical clarity
- Heat resistant, recommended for temperatures from -40°C to +120°C
- Certified DNase, RNase, and nucleic acid free
ThermalSeal RT2RR™ 50 μm polyester sealing films for real-time PCR are sized to fit within the edges of raised-rim 96 well plates. The same consistent ultra-high optical clarity as Excel′s ThermalSeal RT™ sealing films makes possible more reproducible, reliable, and consistent DNA amplification measurements. An inert, strong, temperature-resistant adhesive assures reliable sealing around each well. Two end tabs assist in positioning the sealing film. Easy removal of the end tabs at perforated boundaries prevents lifting and higher evaporation rates that can occur with sealing films that overlap the plate rim. Recommended for specific DNA sequence detection in clinical diagnostics, forensic science and basic research.
Dimensions 77.8 by 130.8 mm. With end tabs removed, length is 118.1 mm with 45° corners.
Dimensions 77.8 by 130.8 mm. With end tabs removed, length is 118.1 mm with 45° corners.
- Ultra-high optical clarity
- Fit within plate rim to prevent evaporation due to sealing film lifting
- Heat resistant, recommended for temperatures from
- -40 °C to +120 °C
- Certified DNase, RNase, and Nucleic Acid free
Legal Information
ThermalSeal is a registered trademark of Excel Scientific, Inc.
ThermalSeal RT is a trademark of Excel Scientific, Inc.
ThermalSeal RT2RR is a trademark of Excel Scientific, Inc.
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Protocols
使用逆转录酶和dNTPs进行逆转录（RT）使用总RNA或基因特异性方法实现仅目标RNA被转化为cDNA。
逆转录是一种旨在测量基因mrna浓度的基因表达分析。 这种分析存在多个挑战，例如不同转录本之间的半衰期差异、时间模式和缺乏相关蛋白。
使用SYBR Green试剂的定量PCR实验方案。该方法支持绝大多数qPCR仪器。
引物浓度优化实验方案是一种建立反应阵列的方法。该阵列用来对照伴侣引物的不同浓度来测试每种引物的一系列浓度。
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