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About This Item
NACRES:
NB.22
UNSPSC Code:
41122410
Manufacturer/tradename:
Excel Scientific TSS-RTQ-100
Material:
polyolefin film, silicone/adhesive
W × l:
7.62 cm × 13.34 cm
Sterility:
non-sterile
Packaging:
pack of 100 ea
material
polyolefin film, silicone/adhesive
sterility
non-sterile
packaging
pack of 100 ea
manufacturer/tradename
Excel Scientific TSS-RTQ-100
technique(s)
PCR: suitable, qPCR: suitable
L × W
76.2 mm × 133.4 mm
W × L
7.62 cm × 13.34 cm
color
clear
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General description
- High optical clarity
- Minimal to no autofluorescence
- Chemically inert; no extractables except at extreme pH
- DMSO resistant for HTS
- Heat resistant, recommended for temperatures from
- -70°C to +100°C
- Certified DNase, RNase, and Nucleic Acid free
- Fit within raised plate rim to prevent loss of seal due to film lifting
- Silicone adhesive forms the strongest available seal for evaporation prevention
- Non-tacky adhesive layer simplifies handling of film prior to sealing
ThermalSeal RTS™ sealing film is a 50 μm thick polyolefin with 50 μm inert encapsulated silicone adhesive. They are optically clear, with low autofluorescence, and are especially suited for real-time qPCR, storage, and protein crystallization applications. The encapsulated silicone adhesive is non-tacky until pressed against the sealing surface, at which time adhesive is released only in sealing areas to form the strongest available heat-resistant seal around each well on the plate. Adhesive on non-sealing areas of the film, such as directly over sample wells, remains encapsulated and inert.
ThermalSeal RTS™ films are sized to fit within the edges of raised-rim 96-well plates. Their consistent high optical clarity makes possible reproducible, reliable, and consistent DNA amplification measurements and crystal detection. Two end tabs assist in positioning the film on the plate, and the non-tacky adhesive surface simplifies handling. Easy removal of the end tabs at perforated boundaries prevents lifting and higher evaporation rates that can occur with films that overlap the plate rim.
Dimensions 76.2 by 133.4 mm. With end tabs removed, length is 113.0 mm.
ThermalSeal RTS™ films are sized to fit within the edges of raised-rim 96-well plates. Their consistent high optical clarity makes possible reproducible, reliable, and consistent DNA amplification measurements and crystal detection. Two end tabs assist in positioning the film on the plate, and the non-tacky adhesive surface simplifies handling. Easy removal of the end tabs at perforated boundaries prevents lifting and higher evaporation rates that can occur with films that overlap the plate rim.
Dimensions 76.2 by 133.4 mm. With end tabs removed, length is 113.0 mm.
Legal Information
ThermalSeal is a registered trademark of Excel Scientific, Inc.
ThermalSeal RTS is a trademark of Excel Scientific, Inc.
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Protocols
使用逆转录酶和dNTPs进行逆转录（RT）使用总RNA或基因特异性方法实现仅目标RNA被转化为cDNA。
逆转录是一种旨在测量基因mrna浓度的基因表达分析。 这种分析存在多个挑战，例如不同转录本之间的半衰期差异、时间模式和缺乏相关蛋白。
使用SYBR Green试剂的定量PCR实验方案。该方法支持绝大多数qPCR仪器。
引物浓度优化实验方案是一种建立反应阵列的方法。该阵列用来对照伴侣引物的不同浓度来测试每种引物的一系列浓度。
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