Skip to Content
Merck

Key Documents

View All Documentation

50484

Methylene Blue solution

suitable for microbiology

Synonym(s):

Ehrlich’s reagent III, Loeffler’s Methylene Blue

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View

About This Item

Empirical Formula (Hill Notation):
C16H18ClN3S
CAS Number:
61-73-4
Molecular Weight:
319.85
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
329757603
MDL number:
MFCD00012111
Beilstein/REAXYS Number:
3641570
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

shelf life

limited shelf life, expiry date on the label

concentration

0.5% in H2O (0.5 g/100 mL)

technique(s)

microbe id | staining: suitable

application(s)

microbiology

suitability

Bacillus spp., Corynebacterium spp., bacteria

SMILES string

[Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C

InChI

1S/C16H18N3S.ClH/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;/h5-10H,1-4H3;1H/q+1;/p-1

InChI key

CXKWCBBOMKCUKX-UHFFFAOYSA-M

General description

Methylene Blue was earlier recognized as a good nuclear stain, the staining solutions are always either aged or alkalized, if not both. More importantly it is an excellent stain for ribonucleic acid, which is the important stainable constituent of bacterial bodies, of lymphocyte and hemoportoal cytoplasm, and of nerve cell tigroid granules. It can also be employed in capsule stain. The capsule stain begins as a negative stain leaving the organisms colorless. The capsule are clearly seen as halo surrounding the bacterial cell. Another staining procedure is performed with nigrosin (Cat. No. 43925).

Other Notes

reagent contains:
methylene blue 0.5 g
distilled water 100 ml

Still not finding the right product?

Explore all of our products under Methylene Blue solution

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Substances Subject to be Notified Names

ishl_notified

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library