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AB5592-50UL Anti-GABA A Receptor α1 Antibody

Detect GABA A Receptor α1 using this Anti-GABA A Receptor α1 Antibody validated for use in IH & WB.

Anti-GABA A Receptor α1 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Neurotransmitters & Receptors Gene Symbol: GABRA1, ECA4, GABA, EJM UniProt Number: P14867
AB5592-50UL
50 µL  
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Overview

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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
RIHC, WBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB5592-50UL
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-GABA A Receptor α1 Antibody
Alternate Names
  • gamma-aminobutyric acid receptor type A alpha1 subunit
References
Product Information
FormatAffinity Purified
Control
  • CONTROL ANTIGEN: Included free of charge with the antibody is 40 μg of control antigen. The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
PresentationAffinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, and 0.025% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
Quality SegmentMQ100
Applications
ApplicationDetect GABA A Receptor α1 using this Anti-GABA A Receptor α1 Antibody validated for use in IH & WB.
Key Applications
  • Immunohistochemistry
  • Western Blotting
Application NotesWestern blot: 1:400-1:1000 using ECL on rat brain membranes. Recognizes a 51kDa band.

Immunohistochemistry on rat brain sections. 4% PFA fixation, frozen sections 1:100-1:200

Dilutions should be made using a carrier protein such as BSA (1-3%)

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenPeptide corresponding to amino acids 1-16 from mouse or rat GABA(A) alpha1 subunit (Swiss-Prot accession number P18504, residues 28-43 of the precursor).
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityRecognizes GABA(A) alpha1. The epitope shares little sequence similarity with alpha5 and alpha2 subunits (respectively, 9/16 and 8/16 residues identical). Does not share homology with any other known proteins.

SPECIES REACTIVITIES: It is expected that the antibody will also work on mouse. The epitope is highly conserved in human and bovine (16/17) and chicken (14/17). Other species have not been tested.
Species Reactivity
  • Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryGABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified.
Gene Symbol
  • GABRA1
  • ECA4
  • GABA
  • EJM
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P14867 # GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.
SIZE: 456 amino acids; 51802 Da
SUBUNIT: Binds UBQLN1 (By similarity). Generally pentameric. There are five types of GABA(A) receptor chains: alpha, beta, gamma, delta, and rho.
SUBCELLULAR LOCATION: Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein.
DISEASE: SwissProt: P14867 # Defects in GABRA1 are a cause of juvenile myoclonic epilepsy (JME) [MIM:606904]. JME is a common epileptic syndrome characterized by afebrile seizures, onset in adolescence (rather than in childhood) and myoclonic jerks. & Defects in GABRA1 are the cause of childhood absence epilepsy type 4 (ECA4) [MIM:611136]. ECA4 is a subtype of idiopathic generalized epilepsy (IGE) characterized by onset at age 6-7 years, frequent absence seizures (several per day) and bilateral, synchronous, symmetric 3-Hz spike waves on EEG. During adolescence, tonic-clonic and myoclonic seizures develop. Absence seizures may either remit or persist into adulthood.
SIMILARITY: SwissProt: P14867 ## Belongs to the ligand-gated ionic channel (TC 1.A.9) family.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size50 µL
Transport Information
Supplemental Information
Specifications
Global Trade ITEM Number
Catalogue Number GTIN
AB5592-50UL 04053252401480

Documentation

Anti-GABA A Receptor α1 Antibody SDS

Title

Safety Data Sheet (SDS) 

Anti-GABA A Receptor α1 Antibody Certificates of Analysis

TitleLot Number
RABBIT ANTI-GABA(A) a1 AFFINITY PURIFIED POLYCLONAL ANTIBODY 2965858
RABBIT ANTI-GABA(A) a1 AFFINITY PURIFIED POLYCLONAL ANTIBODY 2953864
RABBIT ANTI-GABA(A) a1 AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2191053 2191053
RABBIT ANTI-GABA(A) a1 AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2289075 2289075
RABBIT ANTI-GABA(A) alpha1 AFFINITY PURIFIED POLYCLONAL ANTIBODY -
RABBIT ANTI-GABA(A) α1 - 3507282 3507282
RABBIT ANTI-GABA(A) α1 - 3511589 3511589
RABBIT ANTI-GABA(A) α1 AFFINITY PURIFIED POLYCLONAL ANTIBODY 3109814
RABBIT ANTI-GABA(A) α1 AFFINITY PURIFIED POLYCLONAL ANTIBODY 3127686
RABBIT ANTI-GABA(A) α1_2843856 2843856

Data Sheet

Title
RABBIT ANTI-GABA(A) 1 AFFINITY PURIFIED POLYCLONAL ANTIBODY