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ABN1455 Anti-NKX6.2

Anti-NKX6.2, Cat. No. ABN1455, is a highly specific rabbit polyclonal antibody that targets Homeobox protein Nkx-6.2 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-NKX6.2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: NKX6-2, GTX, NKX6B UniProt Number: Q9C056
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ABN1455
100 μg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HIH(P), WBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABN1455
DescriptionAnti-NKX6.2
Alternate Names
  • Homeobox protein NK-6 homolog B
  • Homeobox protein Nkx-6.2
  • NKX6.2
Background InformationHomeobox protein Nkx-6.2 (UniProt Q9C056; also known as Homeobox protein NK-6 homolog B) is encoded by the NKX6-2 (also known as GTX, NKX6B) gene (Gene ID 84504) in human. Mammalian NKX gene products and their orthologs in other species are known to regulators of neuronal cell fate. Both the ventral nervous system defective (vnd) gene product in Drosophila and murine NKX proteins in spinal cord direct progenitors to adopt specific ventral neural identities. In mice, NKX6.2 is expressed in a small subset of ventral neural progenitors in a restricted spatial pattern at the border between the medial and caudal ganglionic eminence (MGE and CGE). NKX6,2 is required for specification of V1 interneurons in the spinal cord and progenitors are found to adopt a more dorsal V0 identity in the absence of NKX6,2. In addition, NKX6.2-positive brain region is shown to give rise to both parvalbumin (PV) and somatostatin (SST) cortical interneurons and in particular be enriched in a subpopulation of SST cortical interneurons that coexpress calretinin (CR).
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality SegmentMQ100
Applications
ApplicationAnti-NKX6.2, Cat. No. ABN1455, is a highly specific rabbit polyclonal antibody that targets Homeobox protein Nkx-6.2 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Immunohistochemistry (Paraffin)
  • Western Blotting
Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected NKX6.2 in human cerebral cortex and kidney tissue sections.

Western Blotting Analysis: 1 µg/mL from a representative lot detected Myc-DDK-tagged human NKX6.2 in 10 µg of lysate from transfected HEK293T cells.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to a C-terminal region sequence of human/mouse/rat NKX6.2.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody detected the exogenously expressed human NKX6.2 in transfected HEK293T cells, while no immunoreactive bands were detected in lysates from untransfected cells. The immunogen sequence lies within the region C-terminal to the Homeobox and is 100% conserved among human, mouse, and rat species.
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Mouse, Rat, Rhesus Macaque based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • NKX6-2
  • GTX
  • NKX6B
Purification MethodAffinity purified.
UniProt Number
Molecular Weight~29 kDa observed. 29.26 kDa (human) and 29.36 kDa (mouse and rat) calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry in human pancreas tissue.

Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected NKX6.2 in human pancreas tissue section.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade ITEM Number
Catalogue Number GTIN
ABN1455 04054839077500