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AB5856-50UL Anti-VAMP-2 Antibody

Anti-VAMP-2 Antibody detects level of VAMP-2 & has been published & validated for use in WB.

Anti-VAMP-2 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Synapse & Synaptic Biology Gene Symbol: VAMP2, VAMP-2, FLJ11460, Synaptobrevin-2, SYB2 UniProt Number: P63027
AB5856-50UL
50 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, RWBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB5856-50UL
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-VAMP-2 Antibody
Alternate Names
  • Vesicle-Associated Membrane Protein 2
  • Synaptobrevin 2
References
Product Information
FormatAffinity Purified
Control
  • CONTROL ANTIGEN: Included free of charge with the antibody is 50 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of PBS. For positive control, in Western blot using 20 ng of protein per Minigel lane. For negative control, preincubate 3 μg of fusion protein with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
PresentationAffinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, 5% sucrose as a stabilizer and 0.025% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min)
Quality SegmentMQ100
Applications
ApplicationAnti-VAMP-2 Antibody detects level of VAMP-2 & has been published & validated for use in WB.
Key Applications
  • Western Blotting
Application NotesWestern blot: 1:1,000 using ECL on rat brain membranes.

Dilutions should be made using a carrier protein such as BSA (1-3%)

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenGST fusion protein corresponding to a cytoplasmic, N-terminal part of rat or mouse VAMP-2 (Accession numbers Q64357, NP 036795).
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityRecognizes VAMP-2 (Vesicle-Associated Membrane Protein 2, Synaptobrevin 2). The epitope does not share homology with any other known proteins.

SPECIES REACTIVITIES: It is expected that the antibody will also react with human, Macaca multata and bovine due to sequence homology (25/26). Other species have not been tested.
Species Reactivity
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. This gene is thought to participate in neurotransmitter release at a step between docking and fusion. The protein forms a stable complex with syntaxin, synaptosomal-associated protein, 25 kD, and synaptotagmin. It also forms a distinct complex with synaptophysin. It is a likely candidate gene for familial infantile myasthenia (FIMG) because of its map location and because it encodes a synaptic vesicle protein of the type that has been implicated in the pathogenesis of FIMG.
Gene Symbol
  • VAMP2
  • VAMP-2
  • FLJ11460
  • Synaptobrevin-2
  • SYB2
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P63027 # Involved in the targeting and/or fusion of transport vesicles to their target membrane.
SIZE: 116 amino acids; 12649 Da
SUBUNIT: Interacts with VAPA and VAPB.
SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Single-pass type IV membrane protein. Note=Neuronal synaptic vesicles.
TISSUE SPECIFICITY: Nervous system and skeletal muscle.
SIMILARITY: SwissProt: P63027 ## Belongs to the synaptobrevin family. & Contains 1 v-SNARE coiled-coil homology domain.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain lyophilized material at -20°C for up to 12 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size50 µL
Transport Information
Supplemental Information
Specifications
Global Trade ITEM Number
Catalogue Number GTIN
AB5856-50UL 04053252407284

Documentation

Anti-VAMP-2 Antibody SDS

Title

Safety Data Sheet (SDS) 

Anti-VAMP-2 Antibody Certificates of Analysis

TitleLot Number
RABBIT ANTI-VAMP-2 PURIFIED, POLYCLONAL ANTIBODY - 2420367 2420367
RABBIT ANTI-VAMP-2 PURIFIED, POLYCLONAL ANTIBODY - 2426495 2426495
RABBIT ANTI-VAMP-2 - 2519208 2519208
RABBIT ANTI-VAMP-2 - 3179972 3179972
RABBIT ANTI-VAMP-2 - 3277226 3277226
RABBIT ANTI-VAMP-2 -2558409 2558409
RABBIT ANTI-VAMP-2 -2803277 2803277
RABBIT ANTI-VAMP-2 -2832911 2832911
RABBIT ANTI-VAMP-2 PURIFIED, POLYCLONAL ANTIBODY 2953963
RABBIT ANTI-VAMP-2 PURIFIED, POLYCLONAL ANTIBODY 2918424
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Data Sheet

Title
RABBIT ANTI-VAMP-2 PURIFIED, POLYCLONAL ANTIBODY