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ABE244-S Anti-monomethyl-Histone H3 (Lys36) Antibody, Trial Size

Anti-monomethyl-Histone H3 (Lys36) Antibody Trial Size is a rabbit polyclonal for detection of Histone H3 monomethylated at lysine 36. Also known as Anti-H3K36me1, this antibody has dot blot (DB) proven specificity & has been validated in WB, IP. Small pack size is ideal for screening applications.

Anti-monomethyl-Histone H3 (Lys36) Antibody, Trial Size MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histones Gene Symbol: HIST1H3A, H3FA, HIST1H3B, H3FL, HIST1H3C, H3FC, HIST1H3D, H3FB, HIST1H3E, H3FD, HIST1H3F, H3FI, HIST1H3G, H3FH, HIST1H3H, H3FK, HIST1H3I, H3FF, HIST1H3J, H3FJ, HIST3H3, H3FT, H3T, MGC126886 UniProt Number: Q16695
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ABE244-S
10 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, H, RWB, IP, DBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABE244-S
DescriptionAnti-monomethyl-Histone H3 (Lys36) Antibody, Trial Size
Alternate Names
  • H3 histone family, member T
  • Histone 3, H3
  • Histone cluster 3, H3
  • H3K36me1
Background InformationHistone H3 is one of four histones that form a core octameric structure that is required for the packaging of DNA into nucleosomes, the most basic level of chromatin arrangement. Histones undergo a number of post-translational modifications (PTM) at their N- and C-terminal tails which can alter chromatin structure and stability, and influence DNA repair and gene expression. Histone H3 undergoes methylation of its lysine and arginine residues, with varying biological outcomes. The lysine 36 residue undergoes mono-, di-, or trimethylation which may be performed by one of many methyltransferase enzymes (SET2, NSD1, NSD2, NSD3, ASH1, SMYD2, SETMAR, KMT3A, and SETD3). Several studies have indicated that methylated lysine 36 residues promote Rpd3S-mediated deacetylation of other histones resulting in gene activation.
References
Product Information
FormatAffinity Purified
Control
  • HeLa acid extract and various recombinant Histone proteins
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality SegmentMQ100
Applications
ApplicationAnti-monomethyl-Histone H3 (Lys36) Antibody Trial Size is a rabbit polyclonal for detection of Histone H3 monomethylated at lysine 36. Also known as Anti-H3K36me1, this antibody has dot blot (DB) proven specificity & has been validated in WB, IP. Small pack size is ideal for screening applications.
Key Applications
  • Western Blotting
  • Immunoprecipitation
  • Dot Blot
Application NotesImmunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated monomethyl-Histone H3 (Lys36) from HeLa acid extract.

Dot Blot Specificity Analysis: Unmodified and various modified Histone peptides (see table) were probed with Anti-monomethyl-Histone H3 (Lys36) (0.5 µg/mL).
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to the N-terminus of human Histone H3 monomethylated at Lys36.
EpitopeMonomethylated Lys36
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThis antibody recognizes the N-terminus of Histone H3 monomethylated at Lys36.
Species Reactivity
  • Mouse
  • Human
  • Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • HIST1H3A
  • H3FA
  • HIST1H3B
  • H3FL
  • HIST1H3C
  • H3FC
  • HIST1H3D
  • H3FB
  • HIST1H3E
  • H3FD
  • HIST1H3F
  • H3FI
  • HIST1H3G
  • H3FH
  • HIST1H3H
  • H3FK
  • HIST1H3I
  • H3FF
  • HIST1H3J
  • H3FJ
  • HIST3H3
  • H3FT
  • H3T
  • MGC126886
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~17 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in HeLa acid extract and various recombinant Histone proteins.

Western Blot Analysis: 0.05 µg/mL of this antibody detected monomethyl-Histone H3 (Lys36) in 10 µg of HeLa acid extract.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size10 µg
Transport Information
Supplemental Information
Specifications
Global Trade ITEM Number
Catalogue Number GTIN
ABE244-S 04053252907548

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Categories

Life Science Research > Antibodies and Assays > Primary Antibodies