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AB3849-I Anti-phospho SMAD2 Antibody (Ser465/467)

Anti-phospho SMAD2 Antibody (Ser465/467) is an antibody against phospho SMAD2 for use in Western Blotting.

Anti-phospho SMAD2 Antibody (Ser465/467) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Transcription Factors Gene Symbol: SMAD2, MADH2, MADR2 UniProt Number: Q15796
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AB3849-I
100 μg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB3849-I
ReplacesAB3849
DescriptionAnti-phospho SMAD2 Antibody (Ser465/467)
Alternate Names
  • Mothers against decapentaplegic homolog 2, Ser465/467 phosphorylated
  • hMAD-2, Ser465/467 phosphorylated
  • hSMAD2, Ser465/467 phosphorylated
  • JV18-1, Ser465/467 phosphorylated
  • MAD homolog 2, Ser465/467 phosphorylated
  • Mad-related protein 2, Ser465/467 phosphorylated
  • Mothers against DPP homolog 2, Ser465/467 phosphorylated
  • SMAD 2, Ser465/467 phosphorylated
  • SMAD family member 2, Ser465/467 phosphorylated
  • Smad2, Ser465/467 phosphorylated
Background InformationMothers against decapentaplegic homolog 2 (UniProt Q15796; also known as hMAD-2, hSMAD2, JV18-1, MAD homolog 2, Mad-related protein 2, Mothers against DPP homolog 2, SMAD 2, SMAD family member 2, Smad2) is encoded by the SMAD2 (also known as MADH2, MADR2) gene (Gene ID 4087) in human. Smad2 is a receptor-regulated intracellular signal transducer and transcriptional modulator activated by transforming growth factor TGF-beta and activin type 1 receptor kinases. In response to TGF-beta signal, smad2 is recruited to the activated TGF-beta receptor through its interaction with the SMAD anchor for receptor activation (SARA) protein, where it is phosphorylated by the TGF-beta receptor. The phosphorylation results in its dissociation from SARA and association with another family member smad4. The smad2-smad4 complex then translocates into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. Smad2 can also be phosphorylated by activin type 1 receptor kinase, and thereby transduce activin-dependent signaling events.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality SegmentMQ100
Applications
ApplicationAnti-phospho SMAD2 Antibody (Ser465/467) is an antibody against phospho SMAD2 for use in Western Blotting.
Key Applications
  • Western Blotting
Biological Information
ImmunogenKLH-conjugated, synthetic phospho-peptide corresponding to residues surrounding Ser465/467 of human Smad2.
EpitopePhospho-Ser465/467.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis antibody detects Smad2 Ser465/467 phosphorylation in TGFbeta-stimulated, but not unstimulated HepG2 cells. The corresponding target phosphoserine residues in the alternatively spliced human Smad2Deltaexon3 (Isoform Short) are pSer435 & pSer437 (UniProt Q15796-2).
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Mouse, Rat, Bovine, Xenopus, Zebrafish, Chicken based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • SMAD2
  • MADH2
  • MADR2
Modifications
  • Phosphorylation
  • Phosphorylation
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~53 kDa. 52.31 kDa (Isoform Long) and 48.96 kDa (Isoform Short; Smad2Deltaexon3) calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected Smad2 Ser465/467 phosphorylation in 10 µg lysate from TGFbeta-stimulated, but not unstimulated, HepG2 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade ITEM Number
Catalogue Number GTIN
AB3849-I 04055977333800

Documentation

Anti-phospho SMAD2 Antibody (Ser465/467) Certificates of Analysis

TitleLot Number
Anti-phospho SMAD2 (Ser465/467) - 3172383 3172383
Anti-phospho SMAD2 (Ser465/467) - 3233096 3233096
Anti-phospho SMAD2 (Ser465/467) - 3384842 3384842
Anti-phospho SMAD2 (Ser465/467) - 3451051 3451051
Anti-phospho SMAD2 (Ser465/467) - 3532294 3532294
Anti-phospho SMAD2 (Ser465/467) - 3753293 3753293
Anti-phospho SMAD2 (Ser465/467) - 3910141 3910141
Anti-phospho SMAD2 (Ser465/467) - 3993464 3993464
Anti-phospho SMAD2 (Ser465/467) - 4140613 4140613
Anti-phospho SMAD2 (Ser465/467) -2730005 2730005
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