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CBL1300 Anti-VCAM-1 Antibody, clone M/K

Anti-VCAM-1 Antibody, clone M/K is an antibody against VCAM-1 for use in FC, IH & IP.

Anti-VCAM-1 Antibody, clone M/K MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Adhesion (CAMs) Gene Symbol: VCAM1, INCAM-100, MGC99561, CD106, DKFZp779G2333, L1CAM UniProt Number: P19320
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CBL1300
500 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
MFC, IHC, IP, FUNCRPurifiedMonoclonal Antibody
Description
Catalogue NumberCBL1300
ReplacesMAB2242
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-VCAM-1 Antibody, clone M/K
Alternate Names
  • CD106
References
Product Information
FormatPurified
Control
  • EOMA and BMS2 endothelial cell lysates
PresentationThe monoclonal is presented in phosphate buffered saline containing 10mM sodium azide.
Quality SegmentMQ100
Applications
ApplicationAnti-VCAM-1 Antibody, clone M/K is an antibody against VCAM-1 for use in FC, IH & IP.
Key Applications
  • Flow Cytometry
  • Immunohistochemistry
  • Immunoprecipitation
  • Affects Function
Application NotesStudies of cell-cell interaction

Cell adhesion assays

Inhibition of lymphopoieisis in Whitlock-Witte long-term marrow cultures

Immunoprecipitation of the VCAM-1 integrin complex

Detection of VCAM-1 cells by immunofluorescence and immunohistochemical staining: acetone or fresh frozen sections work best; although zinc formalin or PLP fixations are reported to work but they have not be verified by Chemicon.

Optimal working dilutions must be determined by the end user.
Biological Information
CloneM/K - 2
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRat
SpecificityRecognizes Murine vascular adhesion molecule (VCAM-1), single chain glycoprotein of Mr 100- kDa (under reducing conditions). VCAM-1 is present on the +/+ 2.4 stromal cell clone, large cells in bone marrow, and a subset of thymic macrophages. The stromal cell antigen is also detectable on the EOMA and BMS2 endothelial cell lines. The antibody reacts with all known isoforms of murine VCAM-1.
IsotypeIgG1κ
Species Reactivity
  • Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Two alternatively spliced transcripts encoding different isoforms have been described for this gene.
Gene Symbol
  • VCAM1
  • INCAM-100
  • MGC99561
  • CD106
  • DKFZp779G2333
  • L1CAM
Purification MethodProtein A Purfied
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P19320 # Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
SIZE: 739 amino acids; 81276 Da
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Expressed on inflamed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflamed tissue.
DOMAIN: SwissProt: P19320 Either the first or the fourth Ig-like C2-type domain is required for VLA4-dependent cell adhesion.
PTM: Sialoglycoprotein.
DISEASE:SwissProt: P19320 # May play an important role in the genesis of atherosclerosis and rheumatoid arthritis.
SIMILARITY: Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsFor use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C.
Packaging Information
Material Size500 µg
Transport Information
Supplemental Information
Specifications
Global Trade ITEM Number
Catalogue Number GTIN
CBL1300 04053252362606

Documentation

Anti-VCAM-1 Antibody, clone M/K MSDS

Title

Safety Data Sheet (SDS) 

Anti-VCAM-1 Antibody, clone M/K Certificates of Analysis

TitleLot Number
RAT ANTI-MOUSE CD 106 (VCAM-1) (UNLABELLED, PURIFIED) - 3538957 3538957
RAT ANTI-MOUSE CD 106 (VCAM-1) (UNLABELLED, PURIFIED) MONOCLONAL ANTIBODY 2931117
RAT ANTI-MOUSE CD 106 (VCAM-1) (UNLABELLED, PURIFIED) MONOCLONAL ANTIBODY 3128869
RAT ANTI-MOUSE CD 106 (VCAM-1) (UNLABELLED, PURIFIED) MONOCLONAL ANTIBODY - 2179338 2179338
RAT ANTI-MOUSE CD 106 (VCAM-1) (UNLABELLED, PURIFIED) MONOCLONAL ANTIBODY - 2318097 2318097
RAT ANTI-MOUSE CD 106 (VCAM-1) (UNLABELLED, PURIFIED) MONOCLONAL ANTIBODY - 2697525 2697525
RAT ANTI-MOUSE CD 106 (VCAM-1) (UNLABELLED, PURIFIED) MONOCLONAL ANTIBODY - 3046723 3046723
RAT ANTI-MOUSE CD 106 (VCAM-1) (UNLABELLED, PURIFIED) MONOCLONAL ANTIBODY - 3890519 3890519
RAT ANTI-MOUSE CD 106 (VCAM-1) (UNLABELLED, PURIFIED) MONOCLONAL ANTIBODY - 3930367 3930367
RAT ANTI-MOUSE CD 106 (VCAM-1) (UNLABELLED, PURIFIED) MONOCLONAL ANTIBODY -2554907 2554907
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