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Merck

B6429

Casein Blocking Buffer 10x

for Northern and Southern blotting, solution

Synonym(s):

10x Blocking Buffer

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About This Item

NACRES:
NA.54
UNSPSC Code:
12161700
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grade

Molecular Biology

Quality Level

form

solution

technique(s)

Southern blotting: suitable

storage temp.

room temp

General description

Casein is a phosphoprotein found in milk. It is classified into four subtypes, including αS1-, αS2-, β-, and κ-caseins.
Sigma’s 10x Blocking Buffer is a casein-based blocking agent routinely used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes.

Application

Casein Blocking Buffer 10x has been used in:

  • western blotting
  • enzyme linked immunosorbent assay (ELISA)
  • immunohistochemistry

Features and Benefits

  • Better blocking performance than dried milk or BSA
  • Easy-to-use concentrate
  • Compatible with neutral and positively-charged membranes
  • Compatible with chemiluminescent and colorimetric detection assays

Preparation Note

Dilute 10x Blocking Buffer with molecular biology grade water before use.


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Warning

Hazard Classifications

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable



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