TOX6
In Vitro Toxicology Assay Kit, Sulforhodamine B based
Synonym(s):
biomass viability assay, sulforhodamine B, total biomass assay
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About This Item
NACRES:
NA.84
UNSPSC Code:
12352207
form
liquid
Quality Level
usage
kit sufficient for 1,000 tests
packaging
pkg of 1 kit
storage condition
dry at room temperature
λmax
565 nm
application(s)
cell analysis
detection
detection method
colorimetric
storage temp.
room temp
General description
The Sulforhodamine B method is simple, accurate, and provides consistent results. This assay system is a means of measuring total biomass by staining cellular proteins with sulforhodamine B.
Application
In Vitro Toxicology Assay Kit, Sulforhodamine B based has been used for spectrophotometric measurement of biomass (viable and non-viable cells) by total protein. It has also been used to study the effect of 17-allylamino-17-demethoxygeldanamycin (17-AAG) on proliferation of the five uveal melanoma cell lines. Absorbance of dye is measured at a wavelength of 565nm.
Biochem/physiol Actions
Dye binds to cellular protein and is then solubilized in base.
signalword
Danger
wgk
WGK 2
hcodes
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Corr. 1A
Storage Class
8A - Combustible corrosive hazardous materials
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