Human STEMCCA Constitutive Polycistronic (OKSM) Lentivirus Reprogramming Kit

The Human STEMCCA Constitutive Polycistronic (OKSM) Lentivirus Kit contains high titer polycistronic (OKSM) lentivirus and Polybrene transfection reagent that have been validated for the generation of human induced pluripotent stem cells from human foreskin fibroblasts (HFFs). The STEMCCA vector is comprised of the transcription factors human Oct-4, Klf4, SOX-2, and c-Myc (OKSM), separated by the self-cleaving 2A peptide and IRES sequences (Sommer CA, 2009). The use of a single polycistronic lentiviral vector significantly improves reprogramming efficiencies and reduces the number of viral integrations.

Tested to confirm the generation of iPS cells from p6 human foreskin fibroblasts. Other cell types have not been tested and thus similar results can not be guaranteed.

1. Human STEMCCA Constitutive (OKSM) Lentivirus: (Part number SCR544-1) Two (2) vials of EF1α-hSTEMCCA (OKSM) Lentivirus (Part number CS204502). Each vial contains 15 µL of high titer lentivirus.

2. Polybrene Transfection Reagent: (Part number TR-1003-50UL) One (1) vial containing 50 µL of 10 mg/mL stock.