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About This Item
Linear Formula:
K2HPO4 · 3H2O
CAS Number:
Molecular Weight:
228.22
NACRES:
NA.25
PubChem Substance ID:
UNSPSC Code:
12161700
EC Number:
231-834-5
MDL number:
Quality Level
product line
ReagentPlus®
assay
≥99.0%
form
powder or crystals
pH
9.2-9.4 (25 °C, 50 g/L)
solubility
water: 100 mg/mL, clear, colorless
storage temp.
room temp
SMILES string
O.O.O.[K+].[K+].OP([O-])([O-])=O
InChI
1S/2K.H3O4P.3H2O/c;;1-5(2,3)4;;;/h;;(H3,1,2,3,4);3*1H2/q2*+1;;;;/p-2
InChI key
XQGPKZUNMMFTAL-UHFFFAOYSA-L
General description
Potassium phosphate solution have very high buffering capacity that is widely used in molecular biology, biochemistry, and chromatography. Potassium phosphate dibasic trihydrate is a key component of potassium phosphate buffer, phosphate-buffered saline (PBS), and phosphate-buffered EDTA (PBE). It is extensively used in the preparation of biological samples for various biological experiments. Potassium phosphate buffer is also used in methods such as equilibrium dialysis, high-performance liquid chromatography (HPLC), and affinity capillary electrophoresis (ACE) to study the characteristics of drug-protein interactions in the body. Potassium phosphate buffer solutions consist of mixtures of the potassium phosphate monobasic and potassium phosphate dibasic forms.
Application
Potassium phosphate dibasic trihydrate has been used in the preparation of potassium phosphate buffer for the resuspension of polyelectrolyte capsules. It has also been used as a buffer during glutaminase‑1 (GLS-1) enzyme assay and GLS-2 enzyme assay.
Legal Information
ReagentPlus is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
13 - Non Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Antoine Desrut et al.
Journal of experimental botany, 71(22), 7301-7315 (2020-08-30)
Plants live in close relationships with complex populations of microorganisms, including rhizobacterial species commonly referred to as plant growth-promoting rhizobacteria (PGPR). PGPR are able to improve plant productivity, but the molecular mechanisms involved in this process remain largely unknown. Using
Michael J Soth et al.
Journal of medicinal chemistry, 63(21), 12957-12977 (2020-10-30)
Inhibition of glutaminase-1 (GLS-1) hampers the proliferation of tumor cells reliant on glutamine. Known glutaminase inhibitors have potential limitations, and in vivo exposures are potentially limited due to poor physicochemical properties. We initiated a GLS-1 inhibitor discovery program focused on
Ala A A Al-Subeihi et al.
Toxicology and applied pharmacology, 283(2), 117-126 (2015-01-01)
The present study aims at predicting the level of formation of the ultimate carcinogenic metabolite of methyleugenol, 1'-sulfooxymethyleugenol, in the human population by taking variability in key bioactivation and detoxification reactions into account using Monte Carlo simulations. Depending on the