CMC0008
STEADY Chemically Competent Cells
Escherichia coli, rod shaped
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UNSPSC Code:
12352200
Biological source:
Escherichia coli
Growth mode:
adherent or suspension
Morphology:
rod shaped
Servicio técnico
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Permítanos ayudarleNombre del producto
STEADY Chemically Competent Cells, for plasmid production using unstable DNA
biological source
Escherichia coli
grade
Molecular Biology
form
buffered aqueous solution
growth mode
adherent or suspension
morphology
rod shaped
technique(s)
microbiological culture: suitable
cell transformation
competent cell type: chemically competent
transformation efficiency: >1 x 108 cfu/μg
shipped in
dry ice
storage temp.
−70°C
General description
The STEADY Chemically Competent Cells are a commonly used strain for cloning sequences that suffer unwanted recombination events in other strains.
Clones with inverted repeats or other sequnces prone to recombination are commonly found in retroviral genes, and require cells such as this product to be propagated stably.
Genotype
recA13 supE44 ara-14 galK2 lacY1 proA2 rpsL20(StrR) xyl-5 λ– leu mtl-1 F– mcrB mrr hsdS20(rB–, mB–)
Clones with inverted repeats or other sequnces prone to recombination are commonly found in retroviral genes, and require cells such as this product to be propagated stably.
Genotype
recA13 supE44 ara-14 galK2 lacY1 proA2 rpsL20(StrR) xyl-5 λ– leu mtl-1 F– mcrB mrr hsdS20(rB–, mB–)
Features and Benefits
- Clone unstable sequences with reliable results
- Available as chemically competent or electrocompetent cells
- Your choice of two packaging formats
- High efficiency: over 1 × 108 cfu/μg (chemi) or 1 × 1010 cfu/μg (electro)
- Excellent value
Other Notes
- STEADY chemically competent cells
- pUC 19 transformation control DNA
- recovery medium for cloning
Clase de almacenamiento
10 - Combustible liquids
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