usage
sufficient for 500 reactions
feature
dNTPs included: no, hotstart: no
packaging
pkg of 500 RXNS
manufacturer/tradename
Roche
technique(s)
RT-qPCR: suitable
input
purified DNA
detection method
probe-based
storage temp.
−20°C
General description
KAPA PROBE FAST One-Step qRT-PCR Master Mix (2X) Universal Kit is a sensitive and convenient solution for real-time PCR using RNA as template. The kit is designed for high-throughput, fast-cycling, one-step RNA quantification using sequence-specific fluorogenic probes. It is compatible with all fluorogenic probe-based technologies, including hybridization probes (e.g., FRET), hydrolysis probes (e.g. TaqMan®) and displacement probes (e.g., molecular beacons)
Application
KAPA PROBE FAST One-Step qRT-PCR Master Mix (2X) Universal has been used for:
- Gene expression analysis
- SNP/mutation analysis
- Gene knockdown validation
- RT-PCR
Features and Benefits
Quick Notes:
- The kit is suitable for all fluorogenic probe-based technologies, including hybridization probes (e.g., FRET), hydrolysis probes (e.g., TaqMan) and displacement probes (e.g., molecular beacons).
- Use only gene-specific primers for one-step qRT-PCR.
- Optimal cDNA synthesis is achieved at 42°C for 5 min.
- 3 min at 95°C is sufficient for RT inactivation and DNA polymerase activation.
- Do not exceed 25 μL reaction volumes
Packaging
Kit includes qPCR Master Mix (2X), KAPA RT Mix (50X), dUTP (10 mM), ROX High Reference Dye (50X) and ROX Low Reference Dye (50X)
Preparation Note
Always ensure that components have been fully thawed and thoroughly mixed before use. The KAPA PROBE FAST qPCR Master Mix may not freeze solidly, even when stored at -20 °C. The KAPA RT Mix is temperature-sensitive, and should be stored at -20 °C and kept on ice during use. ROX reference dye is light-sensitive. Exposure to direct light for an extended period of time will result in loss of fluorescent signal intensity.
Analysis Note
All kit components are subjected to stringent functional quality control, are free of detectable contaminating exo- and endonuclease activity, and meet strict requirements with respect to DNA contamination.
Other Notes
For Research Use Only. Not for use in diagnostic procedures.
Legal Information
TaqMan is a registered trademark of Roche Molecular Systems, Inc.
저장 등급
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash