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NACRES:
NA.32
UNSPSC Code:
12352202
Servicio técnico
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wavelength 595 nm
total test time 2 min
working reagent stability 18-26°C for 2 weeks
dynamic range 50 mg/dL 0.5 g/L
Quality Level
parameter
18-26 °C temperature, 50 μL sample volume
storage temp.
2-8°C
General description
Protein determination is one of the most common operations performed in biochemical research. Total protein kit, micro provides a simple, rapid, and highly sensitive method requiring only 50 μL or less sample.
Application
Total protein kit, micro has been used to determine:
- total protein in the bacterial homogenate by Bradford method
- total protein content by the Bradford microassay from the tissue extracts
- serum total protein
- soluble protein content by the Bradford method from leaves
Colorimetric, Endpoint
Suitable for protein determination for samples of up to 0.5 mg/ml.
Biochem/physiol Actions
Brilliant blue G (Coomassie blue) reacts with protein in an acid-alcohol medium to form a blue-colored protein dye complex. The color, measured at 595 nm, is proportional to the protein concentration.
Total protein kit, micro can be used to determine the concentration of proteins in solution.
signalword
Danger
Hazard Classifications
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 1
target_organs
Eyes,Central nervous system
Clase de almacenamiento
3 - Flammable liquids
flash_point_f
132.8 °F
flash_point_c
56 °C
wgk
WGK 3
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